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Overview

  • Product nameAnti-BubR1 antibodySee all BubR1 primary antibodies ...
  • Description
    Rabbit polyclonal to BubR1
  • Specificityab70173 detects endogenous levels of total BubR1 protein.
  • Tested applicationsWB, ELISA more details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide derived from an internal sequence of human BubR1

  • Positive controlExtracts from HeLa and HepG2 cells all treated with H2O2 (100uM, 30mins).

Properties

Applications

Our Abpromise guarantee covers the use of ab70173 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB WB: 1/500 - 1/1000. Detects a band of approximately 130 kDa (predicted molecular weight: 120 kDa).
ELISA ELISA: 1/10000.

Target

  • FunctionEssential component of the mitotic checkpoint. Required for normal mitosis progression. The mitotic checkpoint delays anaphase until all chromosomes are properly attached to the mitotic spindle. One of its checkpoint functions is to inhibit the activity of the anaphase-promoting complex/cyclosome (APC/C) by blocking the binding of CDC20 to APC/C, independently of its kinase activity. The other is to monitor kinetochore activities that depend on the kinetochore motor CENPE. Required for kinetochore localization of CENPE. Negatively regulates PLK1 activity in interphase cells and suppresses centrosome amplification. Also implicated in triggering apoptosis in polyploid cells that exit aberrantly from mitotic arrest. May play a role for tumor suppression.
  • Tissue specificityHighly expressed in thymus followed by spleen. Preferentially expressed in tissues with a high mitotic index.
  • Involvement in diseaseNote=Defects in BUB1B are associated with tumor formation.
    Defects in BUB1B are the cause of premature chromatid separation trait (PCS) [MIM:176430]. PCS consists of separate and splayed chromatids with discernible centromeres and involves all or most chromosomes of a metaphase. It is found in up to 2% of metaphases in cultured lymphocytes from approximately 40% of normal individuals. When PCS is present in 5% or more of cells, it is known as the heterozygous PCS trait and has no obvious phenotypic effect, although some have reported decreased fertility. Inheritance is autosomal dominant.
    Defects in BUB1B are the cause of mosaic variegated aneuploidy syndrome (MVA) [MIM:257300]. MVA is a severe autosomal recessive developmental disorder characterized by mosaic aneuploidies, predominantly trisomies and monosomies, involving multiple different chromosomes and tissues. The proportion of aneuploid cells varies but is usually more than 25% and is substantially greater than in normal individuals. Affected individuals typically present with severe intrauterine growth retardation and microcephaly. Eye anomalies, mild dysmorphism, variable developmental delay, and a broad spectrum of additional congenital abnormalities and medical conditions may also occur. The risk of malignancy is high, with rhabdomyosarcoma, Wilms tumor and leukemia reported in several cases. MVA is caused by biallelic mutations in the BUB1B gene.
  • Sequence similaritiesBelongs to the protein kinase superfamily. Ser/Thr protein kinase family. BUB1 subfamily.
    Contains 1 BUB1 N-terminal domain.
    Contains 1 protein kinase domain.
  • DomainThe D-box targets the protein for rapid degradation by ubiquitin-dependent proteolysis during the transition from mitosis to interphase.
    The BUB1 N-terminal domain directs kinetochore localization and binding to BUB3.
  • Post-translational
    modifications    Proteolytically cleaved by caspase-3 in a cell cycle specific manner. The cleavage might be involved in the durability of the cell cycle delay. Caspase-3 cleavage is associated with abrogation of the mitotic checkpoint. The major site of cleavage is at Asp-610.
    Acetylation at Lys-250 regulates its degradation and timing in anaphase entry.
    Ubiquitinated. Degradated by the proteasome.
    Sumoylated by SUMO2 and SUMO3. The sumoylation mediates the association with CENPE at the kinetochore.
    Autophosphorylated in vitro. Intramolecular autophosphorylation is stimulated by CENPE. Phosphorylated during mitosis and hyperphosphorylated in mitotically arrested cells. Phosphorylation at Ser-670 and Ser-1043 occurs at kinetochores upon mitotic entry with dephosphorylation at the onset of anaphase.
  • Cellular localizationCytoplasm. Nucleus. Chromosome > centromere > kinetochore. Cytoplasm > cytoskeleton > centrosome. Cytoplasmic in interphase cells. Associates with the kinetochores in early prophase. Kinetochore localization requires BUB1, PLK1 and CASC5.

    • Target information above from: UniProt accession O60566 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
    • Alternative names
        Beta homolg of S. cerevisiae BUB 1 antibodyBeta homolg of S. cerevisiae budding uninhibited by benzimidazoles antibodyBUB 1B antibody
        BUB1 budding uninhibited by benzimidazoles 1 homolog beta antibodyBub1A antibodyBUB1B antibodyBUB1B_HUMAN antibodyBUB1beta antibodyBUBR1 antibodyBudding Uninhibited by Benzimidazoles 1 beta antibodyBudding uninhibited by benzimidazoles 1 homolog beta (yeast) antibodyhBUBR1 antibodyMAD3/BUB1 related protein kinase antibodyMAD3/BUB1-related protein kinase antibodyMAD3L antibodyMitotic checkpoint gene BUB1B antibodyMitotic checkpoint kinase MAD3L antibodyMitotic checkpoint serine/threonine protein kinase BUB1 beta antibodyMitotic checkpoint serine/threonine-protein kinase BUB1 beta antibodyMVA1 antibodyOTTHUMP00000160319 antibodyProtein SSK1 antibodySSK 1 antibodySSK1 antibody
      see all

    Anti-BubR1 antibody images

    • Western blot - BubR1 antibody (ab70173)
      Western blot - BubR1 antibody (ab70173)
      All lanes : Anti-BubR1 antibody (ab70173) at 1/500 dilution

      Lane 1 : extracts from HeLa cells treated with H2O2 (100uM, 30mins),
      Lane 2 : extracts from HepG2 cells treated with H2O2 (100uM, 30mins),
      Lane 3 : extracts from HeLa cells treated with H2O2 (100uM, 30mins), with immunising peptide at 10 µg

      Lysates/proteins at 30 µg per lane.


      Predicted band size : 120 kDa
      Observed band size : 130 kDa (why is the actual band size different from the predicted?)

    References for Anti-BubR1 antibody (ab70173)

    ab70173 has not yet been referenced specifically in any publications.

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