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C Reactive Protein Human ELISA Kit (ab99995)

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Overview

  • Product nameC Reactive Protein Human ELISA KitSee all C Reactive Protein kits ...
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeSandwich
  • Sensitivity
    < 2 pg/ml
  • Range
    2.47 pg/ml - 600 pg/ml
  • Recovery

    > 100 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 106.2 95% - 116%
    Serum 116.7 106% - 127%
    Plasma 102.2 93% - 112%

  • Species reactivity
    Reacts with: Human
  • Product overview

    ab99995 C Reactive Protein Human ELISA (Enzyme- Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human C Reactive Protein in serum, plasma and cell culture supernatants. This assay employs an antibody specific for Human C Reactive Protein coated on a 96-well plate. Standards and samples are pipetted into the wells and C Reactive Protein present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human C Reactive Protein antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of C Reactive Protein bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Notes

    Optimization may be required with urine samples.

  • Tested applicationsELISA more details

Properties

  • RelevanceC Reactive Protein is a major acute phase reactant synthesized primarily in the liver hepatocytes. It is a pentraxin (cyclic pentameric protein) compound of five identical nonglycosylated subunits of 206 amino acids each (m.w. 24 kDa), that are bound noncovalently to form the physiologic CRP molecule (m.w. 117.5 kDa). C Reactive Protein mediates activities associated with preimmune nonspecific host resistance. It is opsonic, an initiator of the classical complement cascade and an activator of monocytes/macrophages. CRP also binds to several nuclear components including chromatin, histones and snRNP, suggesting that it may play a role as a scavenger during cell necrosis. Studies have revealed that among other markers of inflammation, CRP shows the strongest association with cardiovascular events. Many clinical studies demonstrated that coronary mortality among patients with unstable angina and elevated CRP is significantly higher comparing with the patients without elevated CRP. Measurements of C reactive protein (hsCRP) in the patients with ischemic heart disease provide a novel method for detecting individuals at high risk of plaque rupture.
  • Cellular localizationSecreted
    • Alternative names
        C reactive protein pentraxin relatedC-reactive proteinC-reactive protein(1-205)
        CRPMGC149895MGC88244 Pentraxin 1PTX 1PTX1
      see all
  • Database links

      • Applications

      Our Abpromise guarantee covers the use of ab99995 in the following tested applications.

      The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

      Application Notes
      ELISA ELISA

      C Reactive Protein Human ELISA Kit images

      • ELISA - C Reactive Protein Human ELISA Kit (ab99995)
        ELISA - C Reactive Protein Human ELISA Kit (ab99995)

      Protocols

      References for C Reactive Protein Human ELISA Kit (ab99995)

      ab99995 has not yet been referenced specifically in any publications.

      Publishing research using ab99995 ? Please let us know so that we can cite the reference in this datasheet

      Product Wall

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      What is the shelf life of your assay kits?


      The shelf life of each of our kits is indicated in the individual protocol booklet.

      ab99995 may be stored for up to 6 months at 2 to 8°C from the date of shipment. Standard (recombinant protein) should be stored at -20°C or -80°...

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      For all those tests that I explained in previous email, I incubate them over night at 4C.
      and dilution for HRP was once 300 fold (as described in the manual) and also when I ran the test
      in parallel with HRP form albumin kit, I diluted 200 fold, ...

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      The lab sent the following comments and questions. Please let me know if you have the answers to those. I think the lab is trying to understand where exactly the problem might lie.

      But I still think the standard curve should look better than what...

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      For all those tests that I explained in previous email, I incubate them over night at 4C.
      and dilution for HRP was once 300 fold (as described in the manual) and also when I ran the test
      in parallel with HRP form albumin kit, I diluted 200 fold, ...

      Read More

      Thank you so much for the additional information. I'll pass it on to the lab.

      Should they have more suggestions, I will let you know.

      I hope the 2 new kits will work as expected.

      Please let me know if you have further questions.

      I ran few more tests again based on what your lab recommended.

      1- I used another kit with standards and my samples without dilution :
      Result: same situation as previous kit for STD and still no detection for my samples.
      my samples are hig...

      Read More

      Thank you for your emails.

      I apologize that I could not get back to you earlier, but our office was closed over the holidays.

      I am very sorry to hear that your effort and attempts to try to get the kits to work were unsuccessful. I will ...

      Read More

      I confirm our PO 2527 of 11/09/2012.
      So please send us new vial of RP-streptavidin concentrate item.

      Thanks in advance and kind regards

      Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

      I am sorry this product did not perform as stated on the datasheet and for the inconvenience this ha...

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      I was just wondering if there is any progress in your investigation or not yet.
      I appreciate if you please let me know as soon as you can, since I need to test my samples
      as soon as it's possible.

      Thank you for your patience.
      I have heard back from the lab today with the following response:

      "I am not quite sure what exactly the issue is with the standard curve. I plotted the values in a linear regression model and curve looks fine...

      Read More

      antibody vials seem to be empty
      lot GR91493-2 and lot GR95066-1

      Thank you for your phone message.

      I have emailed the lab yesterday but have not yet heard back. I will follow up with them today.

      As for the antibody vial:

      Item F detection antibody comes as a very concentrated, small volume liq...

      Read More

      Dear Colleagues,
      a customer of ours bought your AB99995 lot GR91493-2 (our PO 2527), but she found less HRP-streptavidin concentrate (Item G) than expected.
      In fact she found only 40ul instead of 200ul (300X).
      Can you please send us a new v...

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      Thank you for your message. I am sorry to hear there was a shortage of the HRP-streptavidin concentrate item in the ab99995 kit.

      If this has been purchased in the last 6 months, I will be pleased to provide a replacement of this component. In o...

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      a customer of ours has purchased your kit ab99995 (lot GR79069-3) but after dilution of buffer B, this buffer appears to be "opalescent"!.
      Is this normal? Does the customer can use it? Will the kit work?

      Please note that the custome...

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      Thank you for contacting us.

      This is very normal, the buffer contains proteins which gets precipitated at low temperature. Please keep the buffer at room temperature for few minutes and then vortex it. The white opaque residue will go away.
      <...

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      The two most recent CRP ELISA kits we ordered gave no signal at all with urine samples or the standards in the kit. An earlier kit from a different lot worked as expected.

      Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

      I am sorry this product did not perform as stated on the datasheet and for the inconvenience this ha...

      Read More
      

      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"