C Reactive Protein Human ELISA Kit (ab99995)
- Product nameC Reactive Protein Human ELISA KitSee all C Reactive Protein kits ...
- Tests1 x 96 well plate
- Sample typeCell culture supernatant, Serum, Plasma
- Assay typeSandwich
- Sensitivity< 2 pg/ml
- Range2.47 pg/ml - 600 pg/ml
> 100 %
Sample specific recovery Sample type Average % Range Cell culture supernatant 106.2 95% - 116% Serum 116.7 106% - 127% Plasma 102.2 93% - 112%
- Species reactivityReacts with: Human
- Product overview
ab99995 C Reactive Protein Human ELISA (Enzyme- Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human C Reactive Protein in serum, plasma and cell culture supernatants. This assay employs an antibody specific for Human C Reactive Protein coated on a 96-well plate. Standards and samples are pipetted into the wells and C Reactive Protein present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human C Reactive Protein antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of C Reactive Protein bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Optimization may be required with urine samples.
- Tested applicationsELISA more details
- Storage instructionsStore at -20°C. Please refer to protocols.
Components Identifier 1 x 96 tests 20X Wash Buffer Concentrate Item B 1 x 25ml 300X HRP-Streptavidin Concentrate Item G 1 x 200µl Assay Diluent B 5x concentrated buffer. For detection antibody and HRP- Streptavidin diluent. 1 x 15ml Assay Diluent D Item K 2 x 15ml CRP Microplate (12 strips x 8 wells) coated with anti-human CRP 1 unit CRP Standard Human(recombinant) 2 vials Detection Antibody CRP 2 vials Stop Solution Item I 1 x 8ml TMB One-Step Substrate Reagent Item H 1 x 12ml
- C reactive protein pentraxin relatedC-reactive proteinC-reactive protein(1-205)
- CRPMGC149895MGC88244 Pentraxin 1PTX 1PTX1
Our Abpromise guarantee covers the use of ab99995 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
References for C Reactive Protein Human ELISA Kit (ab99995)
ab99995 has not yet been referenced specifically in any publications.