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Overview

  • Product nameC Reactive Protein Rat ELISA KitSee all C Reactive Protein kits ...
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Urine, Serum, Plasma
  • Assay typeSandwich
  • Sensitivity
    = 0.7 ng/ml
  • Range
    0.78 ng/ml - 50 ng/ml
  • Recovery

    95 %

  • Assay time
    3h 30m
  • Species reactivity
    Reacts with: Rat
  • Product overview

    C Reactive Protein (CRP) is a liver protein composed of five identical nonglycosylated subunits, with a total molecular weight of 105 kDa. CRP has a variety of powerful effects related to immunology, inflammation, and coagulation. High plasma concentration of CRP was associated with increased risk of stroke, myocardial infarction, and peripheral vascular disease. CRP has also been associated with increased risks of fatal coronary events among high-risk male smokers and incident coronary disease among the elderly. Originally used as a marker of acute inflammation, CRP has become a leading candidate as the measure for estimating the inflammatory component of cardiovascular disease risk.

    ab108827 C Reactive Protein Rat ELISA kit is designed for detection of rat CRP in urine and cell culture supernatants. This assay employs a quantitative sandwich enzyme immunoassay technique that measures CRP in 3.5 hours. A polyclonal antibody specific for rat CRP has been pre-coated onto a microplate. CRP in standards and samples is sandwiched by the immobilized antibody and biotinylated polyclonal antibody specific for CRP, which is recognized by a streptavidin-peroxidase conjugate. All unbound material is then washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.

  • Tested applicationsELISA more details

Properties

  • RelevanceC Reactive Protein is a major acute phase reactant synthesized primarily in the liver hepatocytes. It is a pentraxin (cyclic pentameric protein) compound of five identical nonglycosylated subunits of 206 amino acids each (m.w. 24 kDa), that are bound noncovalently to form the physiologic CRP molecule (m.w. 117.5 kDa). C Reactive Protein mediates activities associated with preimmune nonspecific host resistance. It is opsonic, an initiator of the classical complement cascade and an activator of monocytes/macrophages. CRP also binds to several nuclear components including chromatin, histones and snRNP, suggesting that it may play a role as a scavenger during cell necrosis. Studies have revealed that among other markers of inflammation, CRP shows the strongest association with cardiovascular events. Many clinical studies demonstrated that coronary mortality among patients with unstable angina and elevated CRP is significantly higher comparing with the patients without elevated CRP. Measurements of C reactive protein (hsCRP) in the patients with ischemic heart disease provide a novel method for detecting individuals at high risk of plaque rupture.
  • Cellular localizationSecreted
    • Alternative names
        C reactive protein pentraxin relatedC-reactive proteinC-reactive protein(1-205)
        CRPMGC149895MGC88244 Pentraxin 1PTX 1PTX1
      see all
  • Database links

      • Applications

      Our Abpromise guarantee covers the use of ab108827 in the following tested applications.

      The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

      Application Notes
      ELISA ELISA

      C Reactive Protein Rat ELISA Kit images

      • ELISA - C Reactive Protein Rat ELISA Kit (ab108827)
        ELISA - C Reactive Protein Rat ELISA Kit (ab108827)
        Rat C Reactive Protein Standard Curve

      Protocols

      References for C Reactive Protein Rat ELISA Kit (ab108827)

      ab108827 has not yet been referenced specifically in any publications.

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      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"