Overview

  • Product name
    Anti-C Reactive Protein antibody [26D7]
    See all C Reactive Protein primary antibodies
  • Description
    Mouse monoclonal [26D7] to C Reactive Protein
  • Tested applications
    Suitable for: WB, ChIP, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant human protein purified from E.coli

  • Positive control
    • HeLa cell lysate.
  • General notes

    This product was changed from ascites to tissue culture supernatant on 18th September 2017. Lot numbers higher than GR262190 will be from tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly.

Properties

Applications

Our Abpromise guarantee covers the use of ab50861 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 26 kDa (predicted molecular weight: 25 kDa).
ChIP Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.

Target

Images

  • All lanes : Anti-C Reactive Protein antibody [26D7] (ab50861) at 1/2000 dilution

    Lane 1 : HepG2 cell lysate
    Lane 2 : SH-SY5Y cell lysate
    Lane 3 : Jurkat cell lysate
    Lane 4 : HeLa cell lysate
    Lane 5 : Purified CRP from human plasma
    Lane 6 : Plasma from Diabetes mellitus patient
    Lane 7 : Plasma from non-patient


    Predicted band size : 25 kDa
    Observed band size : 26 kDa (why is the actual band size different from the predicted?)

References

This product has been referenced in:
  • Carnuta MG  et al. Dysfunctional high-density lipoproteins have distinct composition, diminished anti-inflammatory potential and discriminate acute coronary syndrome from stable coronary artery disease patients. Sci Rep 7:7295 (2017). Read more (PubMed: 28779156) »
  • Chu CS  et al. Electronegative low-density lipoprotein increases C-reactive protein expression in vascular endothelial cells through the LOX-1 receptor. PLoS One 8:e70533 (2013). WB ; Human . Read more (PubMed: 23950953) »

See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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