Overview

  • Product nameAnti-C Reactive Protein antibody
    See all C Reactive Protein primary antibodies
  • Description
    Mouse polyclonal to C Reactive Protein
  • Tested applicationsWBmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    Vector coding for a partial recombinant fusion protein, corresponding to internal sequence amino acids 72-171 of Mouse C Reactive Protein. Target sequence used to make the antibody: SYATKKNSND ILIFWNKDKQ YTFGVGGAEV RFMVSEIPEA PTHICASWES ATGIVEFWID GKPKVRKSLH KGYTVGPDAS IILGQEQDSY GGDFDAKQSL.

  • General notes


    This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed 12910245; Barry and Johnston PubMed: 9234514). The animal's cells produce the protein, which stimulates the animal's immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.

Properties

Applications

Our Abpromise guarantee covers the use of ab43104 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notesWB:1/1000. Predicted molecular weight: 25 kDa.

    This antibody has been tested in Western blot against an E.coli lysate containing the partial recombinant fusion protein used as an immunogen. We have no data on detection of endogenous protein.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionDisplays several functions associated with host defense: it promotes agglutination, bacterial capsular swelling, phagocytosis and complement fixation through its calcium-dependent binding to phosphorylcholine. Can interact with DNA and histones and may scavenge nuclear material released from damaged circulating cells.
    • Tissue specificityFound in plasma.
    • Sequence similaritiesBelongs to the pentaxin family.
      Contains 1 pentaxin domain.
    • Cellular localizationSecreted.
    • Information by UniProt
    • Database links
    • Alternative names
      • Pentraxin 1, short antibody
      • C reactive protein antibody
      • C reactive protein pentraxin related antibody
      • C-reactive protein(1-205) antibody
      • CRP antibody
      • CRP_HUMAN antibody
      • MGC88244 antibody
      • Pentraxin 1 antibody
      • PTX 1 antibody
      • PTX1 antibody
      see all

    Anti-C Reactive Protein antibody images

    • All lanes : Anti-C Reactive Protein antibody (ab43104) at 1/1000 dilution

      Lane 1 : ~20ug of a total protein extract from E coli, with ~50ng to 100ng of a tagged fusion protein of an irrelevant antigen
      Lane 2 : ~20ug of a total protein extract from E coli, with ~50ng to 500ng of the antigen (tagged fusion protein)

      Secondary
      Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated, at 1/5000 dilution

      Predicted band size : 25 kDa

    References for Anti-C Reactive Protein antibody (ab43104)

    ab43104 has not yet been referenced specifically in any publications.

    Product Wall

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"