Anti-C Reactive Protein antibody (ab43104)

Overview

• Product nameAnti-C Reactive Protein antibodySee all C Reactive Protein primary antibodies ...
• Description
  Mouse polyclonal to C Reactive Protein
• Tested applicationsWB more details
• Species reactivity
  Reacts with: Mouse
  
• Immunogen

  Vector coding for a partial recombinant fusion protein, corresponding to internal sequence amino acids 72-171 of Mouse C Reactive Protein. Target sequence used to make the antibody: SYATKKNSND ILIFWNKDKQ YTFGVGGAEV RFMVSEIPEA PTHICASWES ATGIVEFWID GKPKVRKSLH KGYTVGPDAS IILGQEQDSY GGDFDAKQSL.

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Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
• Storage bufferPreservative: None
  Constituents: 50% Glycerol, Whole serum
• PurityWhole antiserum
• Primary antibody notes This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed 12910245; Barry and Johnston PubMed: 9234514). The animal's cells produce the protein, which stimulates the animal's immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Metabolism
  • Types of disease
  • Metabolic disorders

  • Cardiovascular
  • Atherosclerosis
  • Vascular Inflammation
  • Inflammatory mediators

  • Cardiovascular
  • Heart
  • Apoptosis

  • Cardiovascular
  • Blood
  • Acute Phase Reactants

  • Epigenetics and Nuclear Signaling
  • Chromatin Binding Proteins
  • DNA / RNA binding

  • Immunology
  • Innate Immunity
  • Complement
  • Classical Pathway

  • Immunology
  • Innate Immunity
  • Macrophage / Inflamm.

Applications

Target

• FunctionDisplays several functions associated with host defense: it promotes agglutination, bacterial capsular swelling, phagocytosis and complement fixation through its calcium-dependent binding to phosphorylcholine. Can interact with DNA and histones and may scavenge nuclear material released from damaged circulating cells.
• Tissue specificityFound in plasma.
• Sequence similaritiesBelongs to the pentaxin family.
  Contains 1 pentaxin domain.
• Cellular localizationSecreted.

Target information above from: UniProt accession P02741 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 12944 Mouse
  • SwissProt: P14847 Mouse
  • Unigene: 28767 Mouse

• Alternative names
  C reactive protein antibodyC reactive protein pentraxin related antibodyC-reactive protein(1-205) antibody

  CRP antibodyCRP_HUMAN antibodyMGC88244 antibodyPentraxin 1 antibodyPTX 1 antibodyPTX1 antibody

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Anti-C Reactive Protein antibody images

• 

  Western blot - C Reactive Protein antibody (ab43104)
  All lanes : Anti-C Reactive Protein antibody (ab43104) at 1/1000 dilution
  
  Lane 1 : ~20ug of a total protein extract from E coli, with ~50ng to 100ng of a tagged fusion protein of an irrelevant antigen
  Lane 2 : ~20ug of a total protein extract from E coli, with ~50ng to 500ng of the antigen (tagged fusion protein)
  
  Secondary
  Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated, at 1/5000 dilution
  
  Predicted band size : 25 kDa