Overview
- Product nameAnti-C10orf7 antibody [EPR5860]See all C10orf7 primary antibodies ...
- DescriptionRabbit monoclonal [EPR5860] to C10orf7
- Tested applicationsWB, IP, Flow Cyt more details
- Species reactivityReacts with: Mouse, Rat, Human
- Immunogen
Synthetic peptide corresponding to residues in Human C10orf7 (O75794 ).
- Positive controlHeLa and 293T cell lysates, 293T cells.
- General notesProduced under U.S. Patent No. 5,675,063.
Properties
- FormLiquid
- Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
- Dissociation constant (Kd) Kd = 6.20 x 10-11 M
- Storage bufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
- PurityTissue culture supernatant
- Clonality Monoclonal
- Clone numberEPR5860
- IsotypeIgG
- Research Areas
Applications
Our Abpromise guarantee covers the use of ab124878 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| WB | WB: 1/1000 - 1/10000. Detects a band of approximately 45 kDa (predicted molecular weight: 39 kDa). |
| IP | IP: 1/10 - 1/100. |
| Flow Cyt | Flow Cyt: 1/10 - 1/100. |
Target
- FunctionRequired for S phase entry of the cell cycle.
- Tissue specificityWidely expressed. Expressed in spleen, thymus, prostate, testis, ovary, small intestine, colon and leukocytes with the highest expression in testis.
- Sequence similaritiesBelongs to the CDC123 family.
- Cellular localizationCytoplasm.
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Database links
- Entrez Gene: 8872 Human
- Entrez Gene: 98828 Mouse
- Entrez Gene: 116656 Rat
- SwissProt: O75794 Human
- SwissProt: Q8CII2 Mouse
- SwissProt: Q62834 Rat
- Unigene: 412842 Human
- Unigene: 181490 Mouse
- Unigene: 11096 Rat
see all
Target information above from: UniProt accession
O75794
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
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Alternative names
- C10orf7 antibodyCD123_HUMAN antibodyCDC123 antibody
- Cell division cycle 123 antibodyCell division cycle 123 homolog antibodyCell division cycle protein 123 homolog antibodyD123 antibodyHT 1080 antibodyHT-1080 antibodyProtein D123 antibodyPZ32 antibody
see all
Anti-C10orf7 antibody [EPR5860] images
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![Western blot - Anti-C10orf7 antibody [EPR5860] (ab124878)](http://a.abcam.com/ps/datasheet/images/124/ab124878/C10orf7-Primary-antibodies-ab124878-1.jpg)
Western blot - Anti-C10orf7 antibody [EPR5860] (ab124878)All lanes : Anti-C10orf7 antibody [EPR5860] (ab124878) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : 293T cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size : 39 kDa -
![Flow Cytometry - Anti-C10orf7 antibody [EPR5860] (ab124878)](http://a.abcam.com/ps/datasheet/images/124/ab124878/C10orf7-Primary-antibodies-ab124878-2.jpg)
Flow Cytometry - Anti-C10orf7 antibody [EPR5860] (ab124878)Flow cytometric analysis of permeabilized 293T cells using ab124878 at a dilution of 1/10 (red) or a rabbit IgG (negative)(green). -
](http://a.abcam.com/ps/datasheet/images/124/ab124878/C10orf7-Primary-antibodies-ab124878-3.jpg)
Other-Anti-C10orf7 antibody [EPR5860](ab124878)Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KD
References for Anti-C10orf7 antibody [EPR5860] (ab124878)
ab124878 has not yet been referenced specifically in any publications.
