Recombinant Anti-C1D antibody [EPR11122] (ab166897)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR11122] to C1D
- Suitable for: WB, ICC/IF
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-C1D antibody [EPR11122]
See all C1D primary antibodies -
Description
Rabbit monoclonal [EPR11122] to C1D -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details
Unsuitable for: IHC-P or IP -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Human fetal heart, heart and skeletal muscle lysates. ICC/IF: MCF7 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR11122 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab166897 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/1000 - 1/5000. Predicted molecular weight: 16 kDa.
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ICC/IF |
1/100 - 1/250.
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Notes |
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WB
1/1000 - 1/5000. Predicted molecular weight: 16 kDa. |
ICC/IF
1/100 - 1/250. |
Target
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Function
Plays a role in the recruitment of the RNA exosome complex to pre-rRNA to mediate the 3'-5' end processing of the 5.8S rRNA; this function may include MPHOSPH6. Can activate PRKDC not only in the presence of linear DNA but also in the presence of supercoiled DNA. Can induce apoptosis in a p53/TP53 dependent manner. May regulate the TRAX/TSN complex formation. Potentiates transcriptional repression by NR1D1 and THRB. -
Tissue specificity
Ubiquitous. Expressed at very high levels in the hippocampus, medulla oblongata, mammary gland, thyroid and salivary gland. Expressed at high levels in the fetal; lung, liver and kidney. Expressed at low levels in skeletal muscle, appendix, heart, lung and colon. -
Sequence similarities
Belongs to the C1D family. -
Post-translational
modificationsPhosphorylated by PRKDC. -
Cellular localization
Cytoplasm. Nucleus > nucleolus. EXOSC10 is required for nucleolar localization. - Information by UniProt
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Database links
- Entrez Gene: 10438 Human
- Entrez Gene: 57316 Mouse
- Entrez Gene: 289810 Rat
- Omim: 606997 Human
- SwissProt: Q13901 Human
- SwissProt: O35473 Mouse
- Unigene: 602900 Human
- Unigene: 697447 Human
see all -
Alternative names
- 1110036E10Rik antibody
- AI875855 antibody
- C1D antibody
see all
Images
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All lanes : Anti-C1D antibody [EPR11122] (ab166897) at 1/1000 dilution
Lane 1 : Human heart tissue lysate with NFDM /TBST
Lane 2 : Human skeletal muscle tissue lysate with NFDM /TBST
Lane 3 : Human thyroid tissue lysate with NFDM /TBST
Lane 4 : Hela (Human cervix adenocarcinoma epithelial cell) whole cell lysate with NFDM /TBST
Lane 5 : HT-1080 (Human fibrosarcoma epithelial cell) whole cell lysate with NFDM /TBST
Lane 6 : MDA-MB-231 (Human breast adenocarcinoma epithelial cell) whole cell lysate with NFDM /TBST
Lane 7 : MCF-7 (Human breast adenocarcinoma epithelial cell) whole cell lysate with NFDM /TBST
Lysates/proteins at 20 µg per lane.
Blocking peptides at 5 % per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/20000 dilution
Predicted band size: 16 kDa
Observed band size: 16 kDa
Exposure time: 1 second -
All lanes : Anti-C1D antibody [EPR11122] (ab166897) at 1/1000 dilution
Lane 1 : Human fetal heart lysate
Lane 2 : Human skeletal muscle lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP antibody at 1/2000 dilution
Predicted band size: 16 kDa -
Immunofluorescent analysis of MCF7 cells labeling C1D using ab166897 at 1/100 dilution.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab166897 has not yet been referenced specifically in any publications.