Anti-C1q antibody (ab64632)

Overview

Properties

Applications

Our Abpromise guarantee covers the use of ab64632 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 33 kDa (predicted molecular weight: 26 kDa).Can be blocked with Mouse C1q peptide (ab91203).

Target

  • FunctionC1q associates with the proenzymes C1r and C1s to yield C1, the first component of the serum complement system. The collagen-like regions of C1q interact with the Ca(2+)-dependent C1r(2)C1s(2) proenzyme complex, and efficient activation of C1 takes place on interaction of the globular heads of C1q with the Fc regions of IgG or IgM antibody present in immune complexes.
  • Involvement in diseaseDefects in C1QA are a cause of complement component C1q deficiency (C1QD) [MIM:613652]. A rare defect resulting in C1 deficiency and impaired activation of the complement classical pathway. C1 deficiency generally leads to severe immune complex disease with features of systemic lupus erythematosus and glomerulonephritis.
  • Sequence similaritiesContains 1 C1q domain.
    Contains 1 collagen-like domain.
  • Post-translational
    modifications
    O-linked glycans consist of Glc-Gal disaccharides bound to the oxygen atom of post-translationally added hydroxyl groups.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • C1QA antibody
    • C1QA_HUMAN antibody
    • C1QB antibody
    • C1QC antibody
    • Complement C1q subcomponent subunit A antibody
    • Complement C1q subcomponent subunit B antibody
    • Complement C1q subcomponent subunit C antibody
    • Complement component 1 q subcomponent A chain antibody
    • Complement component 1 q subcomponent alpha polypeptide antibody
    • Complement component C1q A chain antibody
    • Complement component C1q B chain antibody
    • Complement component C1q C chain antibody
    see all

Anti-C1q antibody images

  • Anti-C1q antibody (ab64632) at 1 µg/ml + Plasma (Mouse) Tissue Lysate at 10 µg

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 26 kDa
    Observed band size : 36 + 33 kDa (why is the actual band size different from the predicted?)


    Exposure time : 12 minutes

References for Anti-C1q antibody (ab64632)

ab64632 has not yet been referenced specifically in any publications.

Product Wall

Abreviews
Application Western blot
Loading amount 40 µg
Gel Running Conditions Reduced Denaturing (12%)
Sample Rat Cell lysate - whole cell (Brain tissue lysate)
Specification Brain tissue lysate
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
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Verified customer

Submitted Dec 24 2013

Thank you for contacting us. None of our antibodies have been validated for in vivo or diagnostic use. This C1q antibody in particular (ab64632) contains sodium azide, which can be very toxic in vivo. I hope this helps, please let me kno...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"