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Western blot - C1s antibody (ab66762)
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Immunocytochemistry/ Immunofluorescence - C1s antibody (ab66762)
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Product Name
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C1s antibody
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See all C1s antibodies (7)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to C1s
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Immunogen
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Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human C1s.
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Reacts with
(species key)
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Hu
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Tested applications
(see key)
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ICC/IF, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ICC/IF: Use at a concentration of 1 µg/ml.
WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 90 kDa (predicted molecular weight: 76 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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This antibody gave a positive signal in Human Plasma Total Protein.
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Cellular localization
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Secreted
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Research areas
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Immunology >> Innate Immunity >> Complement >> Classical Pathway
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Relevance
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C1s proenzyme is a glycoprotein produced primarily by hepatocytes but also by other cells. The proenzyme is produced as a single chain polypeptide of 688 aa. Upon activation the peptide is cleaved between R437 and I438 giving the activated enzyme consisting of two disulfide-linked chains, A chain (52.2 kDa) and B chain (27.7 kDa). The Ca dependent catalytic subunit of the C1 complex (C1q, C1r, C1s) comprises of two C1s and two C1r molecules and this serine protease tetramer is responsible for the initial steps in the classical complement pathway, cleavage of C4 and C2.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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0.900 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this C1s antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for C1s antibody images, references, products related to ab66762 and other tools.
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C1s antibody images:
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 Western blot - C1s antibody (ab66762)
C1s antibody (ab66762) at 1 µg/ml + Human Plasma Total Protein Lysate at 10 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 76 kDa
Observed band size : 90 kDa (why is the actual band size different from the predicted?)
Additional bands at : 65 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 3 minutes
The Complement C1s subcomponent protein contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.
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Immunocytochemistry/ Immunofluorescence - C1s antibody (ab66762)
ICC/IF image of ab66762 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab66762, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 100% methanol fixed (5 min) HepG2 and MCF7 cells at 1µg/ml.
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Search PubMed (MEDLINE) for references to C1s
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C1s antibody - more information
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Customer reviews (feedback) regarding C1s antibody |
Customer FAQs regarding C1s antibody |
Protocols for C1s antibody |
Price and availability of products related to C1s antibody |
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C1s antibody - related products:
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C1s antibody - other tools:
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Contact Abcam with a Technical Enquiry about C1s antibody |
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