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Products:Immunology >> Innate Immunity >> Complement >> Classical Pathway
Overview
Product name
Anti-C1s antibody
See all C1s products (11) ...
Description
Rabbit polyclonal to C1s
Specificity
ab72651 detects endogenous levels of fragment of activated C1s resulting from cleavage adjacent to Arg437.
Tested applications
WB, ELISAmore details
Cross reactivity
Reacts with
Human
Immunogen
Synthetic peptide derived from human C1s.
Positive control
Extracts from 293 cells treated with etoposide (25uM, 1hour) and HeLa cells treated with etoposide (25uM, 24hours).
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Immunology >> Innate Immunity >> Complement >> Classical Pathway
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Western blot - C1s antibody (ab72651)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab72651 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 1 Image
WB
ELISA
Application notes
ELISA: 1/20000.
WB: 1/500 - 1/1000. Detects bands of approximately 75 & 51 kDa (predicted molecular weight: 77 & 51 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
C1s B chain is a serine protease that combines with C1q and C1s to form C1, the first component of the classical pathway of the complement system. C1r activates C1s so that it can, in turn, activate C2 and C4.
Involvement in disease
Defects in C1S are the cause of complement component C1s deficiency (C1SD) [MIM:613783]. A rare defect resulting in C1 deficiency and impaired activation of the complement classical pathway. C1 deficiency generally leads to severe immune complex disease with features of systemic lupus erythematosus and glomerulonephritis.
Sequence similarities
Belongs to the peptidase S1 family.
Contains 2 CUB domains.
Contains 1 EGF-like domain.
Contains 1 peptidase S1 domain.
Contains 2 Sushi (CCP/SCR) domains.
Post-translational
modifications
The iron and 2-oxoglutarate dependent 3-hydroxylation of aspartate and asparagine is (R) stereospecific within EGF domains.
Target information above from: UniProt accessionP09871
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- C1 esterase antibody
- C1S antibody
- C1S_HUMAN antibody
see all
Database links
- Entrez Gene: 716 Human
- Omim: 120580 Human
- SwissProt: P09871 Human
- Unigene: 458355 Human
Anti-C1s antibody images:
Western blot - C1s antibody (ab72651)
All lanes : Anti-C1s antibody (ab72651) at 1/500 dilution
Lane 1 : Extracts from 293 cells treated
with etoposide (25uM, 1hour)
Lane 2 : Extracts from HeLa cells treated with
etoposide (25uM, 24hours)
Lane 3 : Extracts from HeLa cells treated with
etoposide (25uM, 24hours) with Cl-peptide at 5 µg
Lysates/proteins at 5 µg per lane.
Predicted band size : 77 , 51 kDa
Observed band size : 51,75 kDa (why is the actual band size different from the predicted?)
Additional bands at : 22 kDa. We are unsure as to the identity of these extra bands.
References for Anti-C1s antibody (ab72651)
ab72651 has not yet been referenced specifically in any publications.
Publishing research using ab72651? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"