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Product name
Anti-C1s antibody
See all C1s products (11) ...
Description
Rabbit polyclonal to C1s
Tested applications
ELISA, WB, IHC-Pmore details
Cross reactivity
Reacts with
Human
Immunogen
Synthetic peptide derived from a region corresponding to internal residues of Human C1s
Positive control
COLO, Jurkat cells and Human breast carcinoma tissue
Properties
Form
Liquid
Storage instructions
Store at -20°C. Stable for 12 months at -20°C
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+),150mM Sodium chloride, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Immunology >> Innate Immunity >> Complement >> Classical Pathway
Applications
Show applications key
Our Abpromise guarantee covers the use of ab92706 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notes
ELISA: 1/40000.
IHC-P: 1/50 - 1/100.
WB: 1/500 - 1/1000. Predicted molecular weight: 77 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
C1s B chain is a serine protease that combines with C1q and C1s to form C1, the first component of the classical pathway of the complement system. C1r activates C1s so that it can, in turn, activate C2 and C4.
Involvement in disease
Defects in C1S are the cause of complement component C1s deficiency (C1SD) [MIM:613783]. A rare defect resulting in C1 deficiency and impaired activation of the complement classical pathway. C1 deficiency generally leads to severe immune complex disease with features of systemic lupus erythematosus and glomerulonephritis.
Sequence similarities
Belongs to the peptidase S1 family.
Contains 2 CUB domains.
Contains 1 EGF-like domain.
Contains 1 peptidase S1 domain.
Contains 2 Sushi (CCP/SCR) domains.
Post-translational
modifications
The iron and 2-oxoglutarate dependent 3-hydroxylation of aspartate and asparagine is (R) stereospecific within EGF domains.
Target information above from: UniProt accessionP09871
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- C1 esterase antibody
- C1S antibody
- C1S_HUMAN antibody
see all
Database links
- Entrez Gene: 716 Human
- Omim: 120580 Human
- SwissProt: P09871 Human
- Unigene: 458355 Human
Anti-C1s antibody images:
Western blot - C1s antibody (ab92706)
All lanes : Anti-C1s antibody (ab92706) at 1/500 dilution
Lane 1 : COLO cells
(without peptide)
Lane 2 : Jurkat cells
(without peptide)
Lane 3 : COLO cells
(with peptide)
Lysates/proteins at 30 µg per lane.
Predicted band size : 77 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - C1s antibody (ab92706)
ab92706 at 1/50 dilution, staining C1s in Human breast carcinoma paraffin-embedded tissue by immunohistochemistry. Right hand image with peptide and left hand image without peptide.
References for Anti-C1s antibody (ab92706)
ab92706 has not yet been referenced specifically in any publications.
Publishing research using ab92706? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"