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Products:Immunology >> Innate Immunity >> Complement >> Classical Pathway
Overview
Product name
Anti-C2 antibody [5C3]
See all C2 products (6) ...
Description
Mouse monoclonal [5C3] to C2
Specificity
This antibody reacts with C2 and a subfraction of C2 believed to be C2b
Tested applications
ELISA, WBmore details
Cross reactivity
Reacts with
Human
Immunogen
Full length native complement component C2 protein isolated from human plasma.
Epitope
Epitope specificity differs from that of ab17452 as determined by inhibition ELISA.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 0.5M Sodium chloride, 0.01M PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Protein A purified
Clonality
Monoclonal
Clone number
5C3
Myeloma
x63-Ag8.653
Isotype
IgG2a
Light chain type
kappa
Research areas
Immunology >> Innate Immunity >> Complement >> Classical Pathway
Applications
Show applications key
Our Abpromise guarantee covers the use of ab17451 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA
WB
Application notes
ELISA: 1/6500.
WB: Use at an assay dependent dilution. Predicted molecular weight: 102 kDa. In Western blotting after SDS-PAGE, this antibody reacts with C2 and a subfraction of C2 believed to be C2b, both under reducing and non-reducing conditions.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Component C2 which is part of the classical pathway of the complement system is cleaved by activated factor C1 into two fragments: C2b and C2a. C2a, a serine protease, then combines with complement factor 4b to generate the C3 or C5 convertase.
Involvement in disease
Defects in C2 are the cause of complement component 2 deficiency (C2D) [MIM:217000]. A deficiency of the complement classical pathway associated with the development of autoimmune disorders, mainly systemic lupus erythematosus. Skin and joint manifestations are common and renal disease is relatively rare. Patients with complement component 2 deficiency are also reported to have recurrent or invasive infections.
Sequence similarities
Belongs to the peptidase S1 family.
Contains 1 peptidase S1 domain.
Contains 3 Sushi (CCP/SCR) domains.
Contains 1 VWFA domain.
Cellular localization
Secreted.
Target information above from: UniProt accessionP06681
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- C2 antibody
- C2 protein antibody
- C3/C5 convertase antibody
see all
Database links
- Entrez Gene: 717 Human
- Omim: 217000 Human
- SwissProt: P06681 Human
- Unigene: 408903 Human
References for Anti-C2 antibody [5C3] (ab17451)
This product has been referenced in:
- Rorbach Jet al. PDE12 removes mitochondrial RNA poly(A) tails and controls translation in human mitochondria. Nucleic Acids Res : (2011). WB; Human.Read more (PubMed: 21666256) »
- Stenbaek EIet al. Human complement component C2: production and characterization of polyclonal and monoclonal antibodies against C2. Mol Immunol 23:879-86 (1986).Read more (PubMed: 3796629) »
See all 2 publications for this product
Publishing research using ab17451? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"