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Product Name
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C3 antibody
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See all C3 antibodies (12)...
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Product type
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Primary antibodies
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Description
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Goat polyclonal to C3
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Immunogen
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Full length native protein (purified) (Human).
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Reacts with
(species key)
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Hu
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Tested applications
(see key)
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DID
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Abreviews
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Application notes
(see key)
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Recommended dilutions
DID: 1/8.
Using an Ouchterlony double diffusion (ODD) assay, in 1% agarose, 5 µl of serially diluted reconstituted antiserum is reacted against 5 µl of 1 mg/ml solution of purified human complement C3 (well separation: 7.5 mm center to center). Titer is equivalent to the highest dilution of antiserum resulting in a visible precipitate after 24 hours.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Cellular localization
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Secreted
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Research areas
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Neuroscience >> Cell Type Marker >> Neuron marker >> Synapse marker
Immunology >> Innate Immunity >> Complement >> Classical Pathway
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Relevance
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The complement factor C3 consists of an alpha and a beta chain. C3 is a central factor in the complement cascade. It is central to the alternative pathway that leads to the C3 convertase C3bBb. The classical mannose binding lectin activation pathway leads to the C3 convertase C4b2a. These convertases cleave C3 resulting in C3a and C3b. Further degradation leads to the formation of the alpha chain products C3d, C3g and C3c. C3 is an acute phase protein that is produced by a wide range of tissues, including renal epithelial cells and hepatocytes.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Goat
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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IgG fraction
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Storage buffer
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Preservative: None
Constituents: 0.1M PBS, pH 7.2
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Purification notes
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The fractionation procedure yields primarily the immunoglobulin fraction of antiserum. The ensure specificity the fractionated antiserum is adsorbed using solid phase techniques, if necessary.
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Form
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Liquid
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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Notes
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Prior to reconstitution store the product at 2-8 °C.
If slight turbidity occurs upon prolonged storage clarify the solution by centrifugation before use.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this C3 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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Search PubMed (MEDLINE) for references to C3
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C3 antibody - more information
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Customer reviews (feedback) regarding C3 antibody |
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