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Purified Human SC5b-9 Complex.
Our Abpromise guarantee covers the use of ab55811 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-Fr||Use at an assay dependent dilution.|
|IHC-P||Use at an assay dependent dilution. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.|
|IHC-FoFr||Use at an assay dependent dilution.|
|RID||Use at an assay dependent dilution.|
|ICC/IF||Use at an assay dependent dilution.|
ab55811 staining C5b-9 (red) in Mouse pancreas tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 5% serum for 1 hour at room temperature; antigen retrieval was by heat mediation. Samples were incubated with primary antibody (1/200 in 5% serum) for 2 hours. An Alexa Fluor®546-conjugated Goat anti-rabbit IgG polyclonal (1/400) was used as the secondary antibody. Insulin - green, DAPI - blue.
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