Anti-C5b-9 antibody (ab55811)

Overview

• Product nameAnti-C5b-9 antibodySee all C5b-9 primary antibodies ...
• Description
  Rabbit polyclonal to C5b-9
• SpecificityThis antibody is monospecific for C5b-9 complex in purified form or present in cobra venom factor activated human serum. There is no reactivity vs. non-activated normal human serum or plasma
• Tested applicationsIHC-Fr, IHC-P, IHC-FoFr, RID, ICC/IF more details
• Species reactivity
  Reacts with: Mouse, Human
  
• Immunogen

  Purified Human SC5b-9 Complex.

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Storage bufferPreservative: 0.1% Sodium Azide
  Constituents: PBS, pH 7.2
• Concentration information loading...
• PurityProtein A purified
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Immunology
  • Innate Immunity
  • Complement
  • Regulatory

  • Immunology
  • Innate Immunity
  • Complement
  • Alternative Pathway

  • Immunology
  • Innate Immunity
  • Complement
  • Classical Pathway

Applications

Target

• RelevanceActivation of the complement system plays a key role in normal inflammatory response to injury but may cause substantial injury when activated inappropriately. The complement system is activated either through the classical (antibody induced) or the alternative (microbial surface, polysacharride induced) pathway, both leading to the formation of the C5b9 complex. Fluid phase binding of the multifunctional glycoprotein S protein (vitronectin) to C5b9 leads to the formation of a cytolytically inactive complex, SC5b9, which is unable to attach to cells.
• Cellular localizationSecreted
• Database links
  • Entrez Gene: 735 Human
  • Entrez Gene: 727 Human
  • Entrez Gene: 729 Human
  • Entrez Gene: 730 Human
  • Entrez Gene: 731 Human
  • SwissProt: P01031 Human
  • SwissProt: P13671 Human
  • SwissProt: P10643 Human

  • SwissProt: P07357 Human
  • SwissProt: P02748 Human

  see all

• Alternative names
  C5 antibodyc5b 9 antibodyC6 antibody

  C7 antibodyC8 antibodyC9 antibodyComplement component 5 antibodyComplement component 6 antibodyComplement component 7 antibodyComplement component 8 antibodyComplement component 9 antibodyMAC antibodyMembrane attack complex antibodyTCC antibodyTerminal complement complex antibody

  see all

Anti-C5b-9 antibody images

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - C5b-9 antibody (ab55811)
  ab55811 staining C5b-9 in human liver.
  Left panel: with primary antibody at 4 ug/ml. Right panel: isotype control.
  Sections were stained using an automated system (DAKO Autostainer Plus ), at room temperature: sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers citrate pH6.1 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
• 

  Immunohistochemistry (Frozen sections) - C5b-9 antibody (ab55811)This image is courtesy of an anonymous abreview.
  ab55811 at a 1/1000 dilution staining C5b-9 in mouse heart tissue by Immunohistochemistry (frozen sections), incubated for 16 hours at 4°C. Acetone fixed. Permeabilized using 1% Triton X-100. Blocked with 10% horse serum + 5% BSA for 1 hour at room temperature. Secondary used at 1/500 dilution polyclonal Goat anti-rabbit IgG conjugated to Alexa Fluor 488.

  See Abreview

• 

  Immunocytochemistry/ Immunofluorescence - C5b-9 antibody (ab55811)
  ICC/IF image of ab55811 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab55811, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.