Overview

  • Product nameAnti-C5b-9 antibody
    See all C5b-9 primary antibodies
  • Description
    Rabbit polyclonal to C5b-9
  • SpecificityThis antibody is monospecific for C5b-9 complex in purified form or present in cobra venom factor activated human serum. There is no reactivity vs. non-activated normal human serum or plasma
  • Tested applicationsIHC-Fr, IHC-P, IHC-FoFr, RID, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Purified Human SC5b-9 Complex.

Properties

Applications

Our Abpromise guarantee covers the use of ab55811 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent dilution.
IHC-P Use at an assay dependent dilution. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
IHC-FoFr Use at an assay dependent dilution.
RID Use at an assay dependent dilution.
ICC/IF Use at an assay dependent dilution.

Target

  • RelevanceActivation of the complement system plays a key role in normal inflammatory response to injury but may cause substantial injury when activated inappropriately. The complement system is activated either through the classical (antibody induced) or the alternative (microbial surface, polysacharride induced) pathway, both leading to the formation of the C5b9 complex. Fluid phase binding of the multifunctional glycoprotein S protein (vitronectin) to C5b9 leads to the formation of a cytolytically inactive complex, SC5b9, which is unable to attach to cells.
  • Cellular localizationSecreted
  • Database links
  • Alternative names
    • C5 antibody
    • c5b 9 antibody
    • C6 antibody
    • C7 antibody
    • C8 antibody
    • C9 antibody
    • Complement component 5 antibody
    • Complement component 6 antibody
    • Complement component 7 antibody
    • Complement component 8 antibody
    • Complement component 9 antibody
    • MAC antibody
    • Membrane attack complex antibody
    • TCC antibody
    • Terminal complement complex antibody
    see all

Anti-C5b-9 antibody images

  • ab55811 staining C5b-9 (red) in Mouse pancreas tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 5% serum for 1 hour at room temperature; antigen retrieval was by heat mediation. Samples were incubated with primary antibody (1/200 in 5% serum) for 2 hours. An Alexa Fluor®546-conjugated Goat anti-rabbit IgG polyclonal (1/400) was used as the secondary antibody. Insulin - green, DAPI - blue.

    See Abreview

  • ab55811 staining C5b-9 in human liver.
    Left panel: with primary antibody at 4 ug/ml. Right panel: isotype control.
    Sections were stained using an automated system (DAKO Autostainer Plus ), at room temperature: sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers citrate pH6.1 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
  • ab55811 at a 1/1000 dilution staining C5b-9 in mouse heart tissue by Immunohistochemistry (frozen sections), incubated for 16 hours at 4°C. Acetone fixed. Permeabilized using 1% Triton X-100. Blocked with 10% horse serum + 5% BSA for 1 hour at room temperature. Secondary used at 1/500 dilution polyclonal Goat anti-rabbit IgG conjugated to Alexa Fluor 488.

    See Abreview

  • ICC/IF image of ab55811 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab55811, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-C5b-9 antibody (ab55811)

This product has been referenced in:
  • Chen C  et al. Role of extracellular RNA and TLR3-Trif signaling in myocardial ischemia-reperfusion injury. J Am Heart Assoc 3:e000683 (2014). IHC-Fr ; Mouse . Read more (PubMed: 24390148) »
  • Tong HH  et al. Deletion of the complement C5a receptor alleviates the severity of acute pneumococcal otitis media following influenza A virus infection in mice. PLoS One 9:e95160 (2014). IHC-P ; Mouse . Read more (PubMed: 24740152) »

See all 14 Publications for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Loading amount 80 µg
Gel Running Conditions Non-reduced Denaturing
Sample Mouse Tissue lysate - whole (Brain)
Specification Brain
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
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Abcam user community

Verified customer

Submitted Jun 23 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: rt°C
Antigen retrieval step None
Sample Mouse Tissue sections (mouse heart)
Specification mouse heart
Permeabilization Yes - 1% triton x-100
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Sep 12 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: rt°C
Antigen retrieval step Heat mediated
Sample Mouse Tissue sections (mouse pancreatic islets)
Specification mouse pancreatic islets
Permeabilization Yes - 1% triton x-100
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Jul 02 2013

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The datasheet pdfs can be downloaded from Abcam website.

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Thank you for contacting Abcam.

We have not tested ab55811 on rat tissue that does mean to say that it will not work, just that we have not tried it.

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