Anti-C9 antibody (ab92690)

Overview

• Product nameAnti-C9 antibodySee all C9 primary antibodies ...
• Description
  Rabbit polyclonal to C9
• Tested applicationsELISA, WB, ICC/IF more details
• Species reactivity
  Reacts with: Human
  

  Does not react with

  Mouse
• Immunogen

  Synthetic peptide derived from an internal region of Human C9.

• Positive controlCOLO cell lysate

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
• Storage bufferPreservative: 0.02% Sodium Azide
  Constituents: 50% Glycerol, PBS (without Mg²⁺ and Ca²⁺), 150mM Sodium chloride, pH 7.4
• Concentration information loading...
• PurityImmunogen affinity purified
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Immunology
  • Innate Immunity
  • Complement
  • MAC

Applications

Target

• FunctionConstituent of the membrane attack complex (MAC) that plays a key role in the innate and adaptive immune response by forming pores in the plasma membrane of target cells. C9 is the pore-forming subunit of the MAC.
• Tissue specificityPlasma.
• Involvement in diseaseDefects in C9 are a cause of complement component 9 deficiency (C9D) [MIM:613825]. A rare defect of the complement classical pathway associated with susceptibility to severe recurrent infections, predominantly by Neisseria gonorrhoeae or Neisseria meningitidis.
• Sequence similaritiesBelongs to the complement C6/C7/C8/C9 family.
  Contains 1 EGF-like domain.
  Contains 1 LDL-receptor class A domain.
  Contains 1 MACPF domain.
  Contains 1 TSP type-1 domain.
• Post-translational
  modificationsThrombin cleaves factor C9 to produce C9a and C9b.
  Phosphorylation sites are present in the extracelllular medium.
• Cellular localizationSecreted. Cell membrane. Secreted as soluble monomer. Oligomerizes at target membranes, forming a pre-pore. A conformation change then leads to the formation of a 100 Angstrom diameter pore.

Target information above from: UniProt accession P02748 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 735 Human
  • Omim: 120940 Human
  • SwissProt: P02748 Human
  • Unigene: 654443 Human

• Alternative names
  C9 antibodyC9 deficiency antibodyC9 deficiency with dermatomyositis antibody

  CO9_HUMAN antibodyComplement component 9 antibodyComplement component 9 deficiency antibodyComplement component C9 antibodyComplement component C9b antibody

  see all

Anti-C9 antibody images

• 

  Western blot - C9 antibody (ab92690)
  All lanes : Anti-C9 antibody (ab92690) at 1/500 dilution
  
  Lane 1 : COLO cell lysate
  Lane 2 : COLO cell lysate with blocking peptide at 10 µg
  
  Lysates/proteins at 30 µg per lane.
  
  
  Predicted band size : 63 kDa
  
• 

  Immunocytochemistry/ Immunofluorescence - Anti-C9 antibody (ab92690)
  ICC/IF image of ab92690 stained HeLa cells. The cells were 4% formaldehyde (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab92690, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.