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ab3982 staining CA19-9 in human colorectal adenocarcinoma by IHC-P (Formalin/Paraformaldehye fixed paraffin embedded tissue sections).
All lanes : Anti-CA19-9 antibody [121SLE] (ab3982) at 5 µg/ml
Lane 2 :
Lane 3 :
Lysates/proteins at 20 µg per lane.
Secondary
Peroxidase- conjugated AffiniPure Goat Anti-mouse IgM at 1/3000 dilution
developed using the ECL technique
Performed under reducing conditions.
Observed band size : 32 kDa (why is the actual band size different from the predicted?)
Additional bands at : 50 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 20 minutes
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