![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - CA19-9 antibody [121SLE] (ab3982) image](#ab3982_1.jpg)
![Western blot - Anti-CA19-9 antibody [121SLE] (ab3982) image](#CA19-9-Primary-antibodies-ab3982-2.jpg)
Loading...
You have changed your country from
to
. Please be aware that this will change the currency in the purchasing process.
Products:Cardiovascular >> Blood >> Blood Cell Antigens
Overview
Product name
Anti-CA19-9 antibody [121SLE]
See all CA19-9 products (7) ...
Description
Mouse monoclonal [121SLE] to CA19-9
Specificity
The antibody recognizes Sialyl Lewis a containing glycolipids.
Tested applications
ELISA, IHC-Pmore details
Cross reactivity
Reacts with
Human
Immunogen
Immunoprecipitate obtained after immunodiffusion of MAb 19-9 and mucins isolated from an ovarian cyst of a 0Le(a+b-) patient.
Positive control
In Western Blot, this antibody gave a positive signal in human breast and human kidney tissue lysates.
Properties
Form
Liquid
Storage instructions
Store at +4°C. Do not freeze.
Storage buffer
Preservative: 15mM Sodium Azide
Constituents: PBS, pH 7.4
Concentration
Concentration information loading...
Purity
>95% by SDS-PAGE
Clonality
Monoclonal
Clone number
121SLE
Isotype
IgM
Research areas
Cardiovascular >> Blood >> Blood Cell Antigens >> RBC Antigens >> Blood Group Antigens
Cancer >> Tumor biomarkers >> Tumor antigens
Tags & Cell Markers >> Cell Type Markers >> Tumor Associated
Cardiovascular >> Blood >> Blood Cell Antigens
Applications
Show applications key
Our Abpromise guarantee covers the use of ab3982 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA
- 1 Image
IHC-P
Application notes
ELISA: Use at an assay dependent dilution.
IHC-P: 1/100 (PMID 20010946).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Alternative names
- Sialyl Lewis (a) antibody
- Sialyl Lewis(a) antibody
- Sialyl Lewisa antibody
Anti-CA19-9 antibody [121SLE] images:
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - CA19-9 antibody [121SLE] (ab3982)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - CA19-9 antibody [121SLE] (ab3982)](/ps/datasheet/Images/3/ab3982/ab3982_1.jpg)
ab3982 staining CA19-9 in human colorectal adenocarcinoma by IHC-P (Formalin/Paraformaldehye fixed paraffin embedded tissue sections).
Western blot - Anti-CA19-9 antibody [121SLE] (ab3982)
![Western blot - Anti-CA19-9 antibody [121SLE] (ab3982)](/ps/datasheet/images/3/ab3982/CA19-9-Primary-antibodies-ab3982-2.jpg)
All lanes : Anti-CA19-9 antibody [121SLE] (ab3982) at 5 µg/ml
Lane 2 : Breast (Human) Tissue Lysate - normal tissue (ab30090)
Lane 3 : Kidney (Human) Tissue Lysate - adult normal tissue (ab30203)
Lysates/proteins at 20 µg per lane.
Secondary
Peroxidase- conjugated AffiniPure Goat Anti-mouse IgM at 1/3000 dilution
developed using the ECL technique
Performed under reducing conditions.
Observed band size : 32 kDa (why is the actual band size different from the predicted?)
Additional bands at : 50 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 20 minutes
References for Anti-CA19-9 antibody [121SLE] (ab3982)
This product has been referenced in:
- Schnegelsberg Bet al. Lectin Histochemistry of Metastasizing and Non-metastasizing Breast and Colon Cancer Cells. Anticancer Res 31:1589-97 (2011).Read more (PubMed: 21617214) »
- Shi CJet al. CD133 gallbladder carcinoma cells exhibit self-renewal ability and tumorigenicity. World J Gastroenterol 17:2965-71 (2011). IHC-P; Human.Read more (PubMed: 21734809) »
See all 5 publications for this product
Publishing research using ab3982? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"