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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-CA19-9 antibody [192](ab25802)
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Product Name
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CA19-9 antibody [192]
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See all CA19-9 antibodies (7)...
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Product type
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Primary antibodies
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Description
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Mouse monoclonal [192] to CA19-9
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Immunogen
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Human colorectal adenocarcinoma cell-line COLO 205.
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Reacts with
(species key)
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Hu
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Specificity
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Reacts specifically with Sialyl Lewisa containing glycolipids (i.e. the CA19-9 epitope), without detectable reactivity with Lewisa or Lewisb or structurally related substances as determined by solid-phase binding assays against glycolipid antigens.
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Tested applications
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IHC-Fr, IHC-P
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Abreviews
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Application notes
(see key)
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Recommended dilutions
IHC-Fr: Use at an assay dependent dilution.
IHC-P: Use at an assay dependent dilution.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Cellular localization
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Cytoplasmic
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Research areas
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Cardiovascular >> Blood >> Blood Cell Antigens >> RBC Antigens >> Blood Group Antigens
Cancer >> Tumor biomarkers >> Tumor antigens
Tags & Cell Markers >> Cell Type Markers >> Tumor Associated
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Relevance
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Mucin glucoprotein is a sialyl Lewisa structure which is synthesized from type 1 blood group precursor chains and is present in individuals expressing the Lewisa and/or Lewisb blood group antigens. In normal tissues, sialyl Lewisa antigen is present in ductal epithelium of the breast, kidney, salivary gland, and sweat glands. Its expression is greatly enhanced in serum as well as in the majority of tumor cells in gastrointestinal (GI) carcinomas.
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Raised in
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Mouse
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Clonality
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Monoclonal
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Clone number
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192
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Myeloma
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Sp2/0
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Isotype
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IgG1
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Purity
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Protein A purified
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Storage buffer
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Preservative: None
Constituents: 0.15M Sodium chloride
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Purification notes
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IgG >98% of total peak area (Superdex 200 gel chromatography on ÄktaFPLC).
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Form
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Liquid
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Concentration
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2.500 mg/ml
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Storage instructions
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Store at +4°C.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this CA19-9 antibody [192] is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for CA19-9 antibody [192] images, references, products related to ab25802 and other tools.
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CA19-9 antibody [192] images:
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 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-CA19-9 antibody [192](ab25802)
Ab25802 staining Human normal kidney medulla. Staining is localized to the cytoplasm.
Left panel: with primary antibody at 1 ug/ml. Right panel: isotype control.
Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffer citrate pH 6.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
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Search PubMed (MEDLINE) for references to CA19-9
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CA19-9 antibody [192] - more information
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Customer reviews (feedback) regarding CA19-9 antibody [192] |
Customer FAQs regarding CA19-9 antibody [192] |
Protocols for CA19-9 antibody [192] |
Price and availability of products related to CA19-9 antibody [192] |
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CA19-9 antibody [192] - related products:
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CA19-9 antibody [192] - other tools:
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Contact Abcam with a Technical Enquiry about CA19-9 antibody [192] |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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