Anti-CABLES1 antibody (ab75535)

Overview

• Product nameAnti-CABLES1 antibodySee all CABLES1 primary antibodies ...
• Description
  Rabbit polyclonal to CABLES1
• Tested applicationsICC/IF, WB, IHC-P more details
• Species reactivity
  Reacts with: Human
  Predicted to work with: Mouse, Zebrafish
• Immunogen

  Synthetic peptide conjugated to KLH derived from within residues 600 to the C-terminus of Human CABLES1.

  Read Abcam's proprietary immunogen policy

  (Peptide available as ab87824.)

• Positive controlThis antibody gave a positive signal in Human Brain and Skeletal Muscle Tissue Lysates, and in the following whole cell lysates: SHSY-5Y; SK N SH; SK N BE; HeLa.

Properties

• FormLiquid
• Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Storage bufferPreservative: 0.02% Sodium Azide
  Constituents: 1% BSA, PBS, pH 7.4
• Concentration information loading...
• PurityImmunogen affinity purified
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Cell Biology
  • Cell Cycle
  • Kinases/Phosphatases
  • Cdk Regulators

Applications

Target

• FunctionCyclin-dependent kinase binding protein. Enhances cyclin-dependent kinase tyrosine phosphorylation by nonreceptor tyrosine kinases, such as that of CDK5 by activated ABL1, which leads to increased CDK5 activity and is critical for neuronal development, and that of CDK2 by WEE1, which leads to decreased CDK2 activity and growth inhibition. Positively affects neuronal outgrowth. Plays a role as a regulator for p53/p73-induced cell death.
• Tissue specificityExpressed in breast, pancreas, colon, head and neck (at protein level). Strongly decreased in more than half of cases of atypical endometrial hyperplasia and in more than 90% of endometrial cancers.
• Sequence similaritiesBelongs to the cyclin family.
• Developmental stageExpression in the endometrial epithelium fluctuates during the menstrual cycle, being greater during the secretory phase when compared with the proliferative phase.
• Post-translational
  modificationsPhosphorylated on Ser-313 by CCNE1/CDK3. Phosphorylated on serine/threonine residues by CDK5 and on tyrosine residues by ABL1. Also phosphorylated in vitro by CCNA1/CDK2, CCNE1/CDK2, CCNA1/CDK3 and CCNE1/CDK3.
• Cellular localizationNucleus. Cytoplasm. Located in the cell body and proximal region of the developing axonal shaft of immature neurons. Located in axonal growth cone, but not in the distal part of the axon shaft or in dendritic growth cone of mature neurons.

Target information above from: UniProt accession Q8TDN4 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 91768 Human
  • Entrez Gene: 63955 Mouse
  • Omim: 609194 Human
  • SwissProt: Q8TDN4 Human
  • SwissProt: Q9ESJ1 Mouse
  • Unigene: 11108 Human
  • Unigene: 40717 Mouse

• Alternative names
  CABL1_HUMAN antibodyCABLES antibodyCables1 antibody

  Cdk5 and Abl enzyme substrate 1 antibodyCDK5 and ABL1 enzyme substrate 1 antibodyFLJ35924 antibodyHsT2563 antibodyIK3 1 antibodyIk3-1 antibodyInteractor with CDK3 1 antibody

  see all

Anti-CABLES1 antibody images

• 

  Western blot - CABLES1 antibody (ab75535)
  All lanes : Anti-CABLES1 antibody (ab75535) at 1 µg/ml
  
  Lane 1 : Brain (Human) Tissue Lysate - adult normal tissue (ab29466)
  Lane 2 : Skeletal Muscle (Human) Tissue Lysate - adult normal tissue (ab29330)
  Lane 3 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate
  Lane 4 : SK N SH (Human neuroblastoma) Whole Cell Lysate
  Lane 5 : SK N BE (Human neuroblastoma) Whole Cell Lysate
  Lane 6 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
  
  Lysates/proteins at 10 µg per lane.
  
  Secondary
  Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
  developed using the ECL technique
  
  Performed under reducing conditions.
  
  Predicted band size : 68 kDa
  Observed band size : 68 kDa
  Additional bands at : 42 kDa (possible isoform).
• 

  Immunocytochemistry/ Immunofluorescence - CABLES1 antibody (ab75535)
  ICC/IF image of ab75535 stained MCF-7 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab75535, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 Goat anti-Rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) HEK293, HepG2 cells at 1µg/ml, and in 100% Methanol fixed (5 min) HeLa, HEK293, HepG2, and MCF-7 cells at 5µg/ml.
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - CABLES1 antibody (ab75535)
  IHC image of CABLES1 staining in Human Breast Carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica Bond^TM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab5535, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.