Anti-CACNA1C antibody (ab81980)
Key features and details
- Goat polyclonal to CACNA1C
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-CACNA1C antibody
See all CACNA1C primary antibodies -
Description
Goat polyclonal to CACNA1C -
Host species
Goat -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Cynomolgus monkey -
Immunogen
Synthetic peptide corresponding to Human CACNA1C aa 2201-2213 (C terminal).
Sequence:C-RARGRPSEEELQD
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Positive control
- Human Heart and Skeletal Muscle lysates.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, Tris buffered saline -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab81980 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab81980 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 0.5 - 1.5 µg/ml. Detects a band of approximately 249 kDa (predicted molecular weight: 249 kDa).
1 hour primary incubation is recommended for this product. |
Notes |
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WB
Use a concentration of 0.5 - 1.5 µg/ml. Detects a band of approximately 249 kDa (predicted molecular weight: 249 kDa). 1 hour primary incubation is recommended for this product. |
Target
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Function
Voltage-sensitive calcium channels (VSCC) mediate the entry of calcium ions into excitable cells and are also involved in a variety of calcium-dependent processes, including muscle contraction, hormone or neurotransmitter release, gene expression, cell motility, cell division and cell death. The isoform alpha-1C gives rise to L-type calcium currents. Long-lasting (L-type) calcium channels belong to the 'high-voltage activated' (HVA) group. They are blocked by dihydropyridines (DHP), phenylalkylamines, benzothiazepines, and by omega-agatoxin-IIIA (omega-Aga-IIIA). They are however insensitive to omega-conotoxin-GVIA (omega-CTx-GVIA) and omega-agatoxin-IVA (omega-Aga-IVA). Calcium channels containing the alpha-1C subunit play an important role in excitation-contraction coupling in the heart. The various isoforms display marked differences in the sensitivity to DHP compounds. Binding of calmodulin or CABP1 at the same regulatory sites results in an opposit effects on the channel function. -
Tissue specificity
Expressed in brain, heart, jejunum, ovary, pancreatic beta-cells and vascular smooth muscle. Overall expression is reduced in atherosclerotic vascular smooth muscle. -
Involvement in disease
Timothy syndrome
Brugada syndrome 3 -
Sequence similarities
Belongs to the calcium channel alpha-1 subunit (TC 1.A.1.11) family. CACNA1C subfamily. -
Domain
Each of the four internal repeats contains five hydrophobic transmembrane segments (S1, S2, S3, S5, S6) and one positively charged transmembrane segment (S4). S4 segments probably represent the voltage-sensor and are characterized by a series of positively charged amino acids at every third position.
Binding of intracellular calcium through the EF-hand motif inhibits the opening of the channel. -
Post-translational
modificationsPhosphorylation by PKA activates the channel. -
Cellular localization
Membrane. Cell membrane. The interaction between RRAD and CACNB2 regulates its trafficking to the cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 775 Human
- Omim: 114205 Human
- SwissProt: Q13936 Human
- Unigene: 118262 Human
- Unigene: 690010 Human
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Alternative names
- alpha-1 polypeptide antibody
- cardiac muscle antibody
- isoform 1 antibody
see all
Images
Datasheets and documents
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Datasheet download
References (3)
ab81980 has been referenced in 3 publications.
- Papp R et al. Genomic upregulation of cardiac Cav1.2a and NCX1 by estrogen in women. Biol Sex Differ 8:26 (2017). PubMed: 28807015
- Papp R et al. Genomic upregulation of cardiac Cav1.2a and NCX1 by estrogen in women. Biol Sex Differ 8:26 (2017). PubMed: 28815011
- Herraiz-Martínez A et al. Ageing is associated with deterioration of calcium homeostasis in isolated human right atrial myocytes. Cardiovasc Res 106:76-86 (2015). PubMed: 25712961