CACNA1H antibody [S55-10] (ab84815)

Product Name 

CACNA1H antibody [S55-10]

Product type 

Primary antibodies

Description 

Mouse monoclonal [S55-10] to CACNA1H

Immunogen 

Fusion protein, corresponding to amino acids 1019-1293 (II-III loop) of Human CACNA1H (accession number O95180)

Reacts with 
(species key)

Hu

Specificity 

No cross-reactivity against Cav1.3

Tested applications 
(see key)

ICC/IF, IP, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
ICC/IF: Use at a concentration of 0.1 - 1 µg/ml. (Perox), 1 - 10 µg/ml (IF).
IP: Use at an assay dependent dilution.
WB: Use at a concentration of 1 - 10 µg/ml. Detects a band of approximately 260 kDa.


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

HEK cell lysate expressing Cav1.3

Cellular localization 

Membrane; Multi pass membrane protein.

Research areas 

Signal Transduction >> Signaling Pathway >> Calcium Signaling >> Calcium Channels
Neuroscience >> Neurotransmission >> Calcium Signaling >> Calcium Channels >> P / Q / R -Type

Relevance 

Voltage-sensitive calcium channels (VSCC) mediate the entry of calcium ions into excitable cells and are also involved in a variety of calcium-dependent processes, such as muscle contraction, hormone or neurotransmitter release, gene expression, cell motility, cell division and cell death. The alpha 1H isoform CACNA1H is a T-type calcium channel and thus belongs to the "low-voltage activated (LVA)" group and is strongly blocked by nickel and mibefradil. A particularity of this type of channels is an opening at quite negative potentials, and a voltage-dependent inactivation. T-type channels exhibit pacemaking functions in both central neurons and cardiac nodal cells, and also function in calcium signaling in secretory cells and vascular smooth muscle. They may also be involved in the modulation of firing patterns of neurons, which is important for information processing as well as in cell growth processes. Mutations in the CACNA1H gene are associated with susceptibility to idiopathic generalized epilepsy type 6 (IGE6).

Database links 

Raised in 

Mouse

Clonality 

Monoclonal

Clone number 

S55-10

Isotype 

IgG1

Purity 

Protein G purified

Storage buffer 

Preservative: 0.09% Sodium Azide
Constituents: 50% Glycerol, PBS, pH 7.4

Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.

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