CACNA1H antibody (ab74656)

Product Name 

CACNA1H antibody

Product type 

Primary antibodies

Description 

Rabbit polyclonal to CACNA1H

Immunogen 

Synthetic peptide derived from an internal sequence of human CACNA1H.

Reacts with 
(species key)

Hu, Ms, Rat

Specificity 

ab74656 detects endogenous levels of total CACNA1H protein.

Epitope 

GB02/02/10 : The immunogen sequence is from 471aa to 500aa

Tested applications 
(see key)

ELISA, WB

Abreviews 

Customer reviews (see key)

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Application notes 
(see key)

Recommended dilutions
ELISA: 1/10000.
WB: 1/500 - 1/1000. Predicted molecular weight: 259 kDa.

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

A549 cell extracts.

Cellular localization 

Membrane; Multi pass membrane protein.

Research areas 

Signal Transduction >> Signaling Pathway >> Calcium Signaling >> Calcium Channels
Neuroscience >> Neurotransmission >> Calcium Signaling >> Calcium Channels >> P / Q / R -Type

Relevance 

Voltage-sensitive calcium channels (VSCC) mediate the entry of calcium ions into excitable cells and are also involved in a variety of calcium-dependent processes, such as muscle contraction, hormone or neurotransmitter release, gene expression, cell motility, cell division and cell death. The alpha 1H isoform CACNA1H is a T-type calcium channel and thus belongs to the "low-voltage activated (LVA)" group and is strongly blocked by nickel and mibefradil. A particularity of this type of channels is an opening at quite negative potentials, and a voltage-dependent inactivation. T-type channels exhibit pacemaking functions in both central neurons and cardiac nodal cells, and also function in calcium signaling in secretory cells and vascular smooth muscle. They may also be involved in the modulation of firing patterns of neurons, which is important for information processing as well as in cell growth processes. Mutations in the CACNA1H gene are associated with susceptibility to idiopathic generalized epilepsy type 6 (IGE6).

Database links 

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg²⁺ and Ca²⁺), 150mM Sodium chloride, pH 7.4

Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS

Purification notes 

ab74656 was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen.

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

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