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Our Abpromise guarantee covers the use of ab3349 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at an assay dependent concentration.
Immunoprecipitation of CAIN from rat brain tissues shows an interaction between CAIN and amphiphysin 1, adaptin, and dynamin 1.
By Western blot, this antibody detects an ~240 kDa protein representing cain from rat liver extract.
|ICC/IF||Use at an assay dependent concentration.|
ICC/IF image of ab3349 stained PC12 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab3349, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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