Anti-CAIN antibody (ab3349)

Overview

• Product nameAnti-CAIN antibodySee all CAIN primary antibodies ...
• Description
  Rabbit polyclonal to CAIN
• Tested applicationsELISA, IP, WB, ICC/IF more details
• Species reactivity
  Reacts with: Rat, Human
  
• Immunogen

  Immunizing peptide is a His-tagged fusion protein corresponding to amino acid residues 1881-2173 from rat CAIN.

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Storage bufferPBS with 0.05% sodium azide
• PurityWhole antiserum
• Primary antibody notes Calcineurin is a widely distributed Ser/Thr, calcium and calmodulin-dependent protein phosphatase. It mediates the immunosuppressive actions of drugs such as FK506 and cyclosporin, and has also been implicated in a number of calcium-sensitive pathways in the nervous system. Calcineurin has been found to be associated with other proteins, such as calmodulin, FKBP12, the ryanodine receptor, inositol 1,4,5-trisphosphate, and a recently identified protein, calcineurin inhibitor (CAIN). CAIN has been shown to bind calcineurin through its C-terminal tail. Recent studies have shown CAIN to bind to both amphiphysin 1 and calcineurin simultaneously and therefore acts as a physiological inhibitor of calcineurin. The binding of CAIN to amphiphysin 1 does not affect the interaction of amphiphysin 1 with other endocytic proteins. CAIN, like calcineurin, is highly expressed in neurons and other tissues.
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Signal Transduction
  • Protein Phosphorylation
  • Ser / Thr Phosphatases

Applications

Target

• RelevanceCalcineurin is a widely distributed Ser/Thr, calcium and calmodulin-dependent protein phosphatase. It mediates the immunosuppressive actions of drugs such as FK506 and cyclosporin, and has also been implicated in a number of calcium-sensitive pathways in the nervous system. Calcineurin has been found to be associated with other proteins, such as calmodulin, FKBP12, the ryanodine receptor, inositol 1,4,5-trisphosphate, and a recently identified protein, calcineurin inhibitor (CAIN). CAIN has been shown to bind calcineurin through its C-terminal tail. Recent studies have shown CAIN to bind to both amphiphysin 1 and calcineurin simultaneously and therefore acts as a physiological inhibitor of calcineurin. The binding of CAIN to amphiphysin 1 does not affect the interaction of amphiphysin 1 with other endocytic proteins. CAIN, like calcineurin, is highly expressed in neurons and other tissues.
• Cellular localizationNuclear
• Database links
  • Entrez Gene: 23523 Human
  • Entrez Gene: 23523 Human
  • Entrez Gene: 94165 Rat
  • Omim: 604251 Human
  • SwissProt: Q9Y6J0 Human
  • SwissProt: Q9Y6J0 Human
  • SwissProt: O88480 Rat
  • Unigene: 517478 Human

  • Unigene: 4 Rat

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• Alternative names
  A330070M20Rik antibodyCabin 1 antibodyCabin1 antibody

  Calcineurin binding protein cabin 1 antibodyCalcineurin inhibitor antibodyKIAA0330 antibodyPPP3IN antibody

  see all

Anti-CAIN antibody images

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  Immunocytochemistry/ Immunofluorescence - CAIN antibody (ab3349)
  ICC/IF image of ab3349 stained PC12 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab3349, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.