Overview

  • Product nameAnti-CAIN antibodySee all CAIN primary antibodies ...
  • Description
    Rabbit polyclonal to CAIN
  • Tested applicationsELISA, IP, WB, ICC/IF more details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Fusion protein (His-tag) corresponding to Rat CAIN aa 1881-2173.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.05% Sodium azide
    Constituent: 99% PBS
  • PurityWhole antiserum
  • Primary antibody notes Calcineurin is a widely distributed Ser/Thr, calcium and calmodulin-dependent protein phosphatase. It mediates the immunosuppressive actions of drugs such as FK506 and cyclosporin, and has also been implicated in a number of calcium-sensitive pathways in the nervous system. Calcineurin has been found to be associated with other proteins, such as calmodulin, FKBP12, the ryanodine receptor, inositol 1,4,5-trisphosphate, and a recently identified protein, calcineurin inhibitor (CAIN). CAIN has been shown to bind calcineurin through its C-terminal tail. Recent studies have shown CAIN to bind to both amphiphysin 1 and calcineurin simultaneously and therefore acts as a physiological inhibitor of calcineurin. The binding of CAIN to amphiphysin 1 does not affect the interaction of amphiphysin 1 with other endocytic proteins. CAIN, like calcineurin, is highly expressed in neurons and other tissues.
  • Clonality Polyclonal
  • IsotypeIgG
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab3349 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
ELISA 1/15000.
IP Use at an assay dependent concentration. Immunoprecipitation of cain from rat brain tissues shows an interaction between CAIN and amphiphysin 1, adaptin, and dynamin 1.
WB 1/1000.

By Western blot, this antibody detects an ~240 kDa protein representing cain from rat liver extract.

ICC/IF Use at an assay dependent concentration.

Target

  • FunctionMay be required for replication-independent chromatin assembly. May serve as a negative regulator of T-cell receptor (TCR) signaling via inhibition of calcineurin. Inhibition of activated calcineurin is dependent on both PKC and calcium signals.
  • Tissue specificityWidely expressed in different tissues.
  • Sequence similaritiesContains 6 TPR repeats.
  • Post-translational
    modifications
    Activated through PKC-mediated hyperphosphorylation.
  • Cellular localizationNucleus.
  • Target information above from: UniProt accession Q9Y6J0 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
  • Alternative names
    • A330070M20Rik antibody
    • Cabin 1 antibody
    • CABIN_HUMAN antibody
    • Cabin1 antibody
    • CAIN antibody
    • Calcineurin binding protein cabin 1 antibody
    • Calcineurin inhibitor antibody
    • Calcineurin-binding protein cabin-1 antibody
    • KIAA0330 antibody
    • PPP3IN antibody
    see all

Anti-CAIN antibody images

  • ICC/IF image of ab3349 stained PC12 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab3349, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-CAIN antibody (ab3349)

This product has been referenced in:
  • Ying CY  et al. MEF2B mutations lead to deregulated expression of the oncogene BCL6 in diffuse large B cell lymphoma. Nat Immunol 14:1084-92 (2013). Read more (PubMed: 23974956) »
  • Rai TS  et al. Human CABIN1 is a functional member of the human HIRA/UBN1/ASF1a histone H3.3 chaperone complex. Mol Cell Biol 31:4107-18 (2011). WB, ICC/IF, IP ; Human . Read more (PubMed: 21807893) »

See all 2 Publications for this product

Product Wall

Application Western blot
Gel Running Conditions Reduced Denaturing (4%-20%)
Sample Mouse Tissue lysate - whole (Whole Embryo)
Specification Whole Embryo
Blocking step Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
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Verified customer

Submitted Aug 13 2013


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Thank you for contacting us. As discussed on the phone this antibody stock is whole serum it is therefore difficult to determine the concentration of the antibody, since whole serum contains many other host serum proteins besides the antibody of intere...

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Thank you for contacting us. We are sorry; ab57137 is no longer included in our product range. We apologize for the inconvenience caused. Could we recommend few other products from our catalogue? We have ab3349 and ab76600 antibodies available ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"