Anti-NDP52 antibody (ab151256)
Key features and details
- Rabbit polyclonal to NDP52
- Suitable for: WB, IHC-P, ICC/IF, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-NDP52 antibody
See all NDP52 primary antibodies -
Description
Rabbit polyclonal to NDP52 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IF, IPmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein corresponding to Human NDP52 aa 174-389.
Database link: Q13137 -
Positive control
- IHC-P: H441 xenograft. WB: Raji cells, Hu7 whole cell extract. ICC/IF: HeLa cells. IP: Jurkat cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab151256 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/10000. Predicted molecular weight: 52 kDa.
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IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Alternatively Tris-EDTA buffer ( pH8.0) may be used.
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ICC/IF |
1/100 - 1/1000.
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IP |
1/100 - 1/500.
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Notes |
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WB
1/500 - 1/10000. Predicted molecular weight: 52 kDa. |
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Alternatively Tris-EDTA buffer ( pH8.0) may be used. |
ICC/IF
1/100 - 1/1000. |
IP
1/100 - 1/500. |
Target
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Function
May play a role in ruffle formation and actin cytoskeleton organization. Seems to negatively regulate constitutive secretion. -
Tissue specificity
Expressed in all tissues tested with highest expression in skeletal muscle and lowest in brain. -
Cellular localization
Cytoplasm > perinuclear region. Golgi apparatus. Cytoplasm > cytoskeleton. According to PubMed:7540613, localizes to nuclear dots. According to PubMed:9230084 and PubMed:12869526, it is not a nuclear dot-associated protein but localizes predominantly in the cytoplasm with a coarse-grained distribution preferentially close to the nucleus. - Information by UniProt
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Database links
- Entrez Gene: 10241 Human
- Omim: 604587 Human
- SwissProt: Q13137 Human
- Unigene: 514920 Human
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Alternative names
- Antigen nuclear dot 52 kDa protein antibody
- CACO2_HUMAN antibody
- Calcium binding and coiled coil domain 2 antibody
see all
Images
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NDP52 was immunoprecipitated from Jurkat whole cell extracts with 5 μg ab151256. Western blot was performed on the immunoprecipitate using ab151256. EasyBlot anti-rabbit IgG was used as the secondary antibody.
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All lanes : Anti-NDP52 antibody (ab151256) at 1/2000 dilution
Lane 1 : Untreated Huh-7 whole cell extracts
Lane 2 : Huh-7 whole cell extracts treated with Thapsigargin for 12hrs
Lane 3 : Huh-7 whole cell extracts treated with Thapsigargin for 24hrs
Lysates/proteins at 30 µg per lane.
Predicted band size: 52 kDa10% SDS-PAGE.
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HeLa stained for NDP52 (green) using ab151256 at 1/1000 dilution in ICC/IF.
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Immunohistochemical analysis of paraffin embedded H441 xenograft labeling NDP52 with ab151256 antibody at 1/100.
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Anti-NDP52 antibody (ab151256) at 1/1000 dilution + Raji whole cell lysate at 30 µg
Predicted band size: 52 kDa
10% SDS PAGE
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab151256 has not yet been referenced specifically in any publications.