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All lanes : Anti-CALML3 antibody (ab72570) at 1/500 dilution
Lane 1 : CALML3 transfected 293T cell lysate
Lane 2 : Non transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Secondary
Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
Predicted band size : 17 kDa
Observed band size : 13 kDa (why is the actual band size different from the predicted?)
Purified ab72570 on formalin-fixed paraffin-embedded HeLa cell at 10 ug/ml
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