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Products:Tags & Cell Markers >> Cell Type Markers >> Epi / Endo-thelial
Overview
Product name
Anti-CALML3 antibody
See all CALML3 products (3) ...
Description
Mouse polyclonal to CALML3
Tested applications
WB, ICC/IFmore details
Cross reactivity
Reacts with
Human
Immunogen
Full length human CALML3 protein (NP_005176.1).
Positive control
WB: A-431 cell lysate and CALML3 transfected 293T cell lysate ICC/IF: HeLa cell lysate
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 1X PBS, pH 7.2
Concentration
Concentration information loading...
Purity
Protein A purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Signal Transduction >> Protein Trafficking >> Chaperones >> Other Chaperones
Signal Transduction >> Signaling Pathway >> Calcium Signaling >> Calcium Binding Proteins
Signal Transduction >> Signaling Pathway >> Calcium Signaling >> Calmodulin Pathway
Tags & Cell Markers >> Cell Type Markers >> Epi / Endo-thelial
Applications
Show applications key
Our Abpromise guarantee covers the use of ab88761 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 1 µg/mlPredicted molecular weight: 17 kDa.
ICC/IF: Use a concentration of 10 µg/ml
Target
Function
May be similar to that of authentic calmodulin and may actually compete with calmodulin by binding, with different affinities, to cellular substrates.
Tissue specificity
Expressed in normal mammary, prostate, cervical, and epidermal tissues. It is greatly reduced or undetectable in transformed cells.
Sequence similarities
Belongs to the calmodulin family.
Contains 4 EF-hand domains.
Target information above from: UniProt accessionP27482
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- CALL3_HUMAN antibody
- CALML3 antibody
- Calmodulin like 3 antibody
see all
Database links
- Entrez Gene: 810 Human
- Omim: 114184 Human
- SwissProt: P27482 Human
- Unigene: 239600 Human
Anti-CALML3 antibody images:
Western blot - CALML3 antibody (ab88761)
Anti-CALML3 antibody (ab88761) at 1 µg/ml + A-431 cell lysate at 50 µg
Predicted band size : 17 kDa
Observed band size : 14 kDa (why is the actual band size different from the predicted?)
Western blot - CALML3 antibody (ab88761)
All lanes : Anti-CALML3 antibody (ab88761) at 1 µg/ml
Lane 1 : CALML3 transfected 293T cell lysate
Lane 2 : Non transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Predicted band size : 17 kDa
Observed band size : 14 kDa (why is the actual band size different from the predicted?)
Immunocytochemistry/ Immunofluorescence - CALML3 antibody (ab88761)
ab88761, at 10 µg/ml, staining CALML3 in HeLa cell by immunofluorescence.
References for Anti-CALML3 antibody (ab88761)
ab88761 has not yet been referenced specifically in any publications.
Publishing research using ab88761? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"