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Products:Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> MAPK Pathway
Overview
Product name
Anti-CAMKK2 antibody - N-terminal
See all CAMKK2 products (4) ...
Description
Rabbit polyclonal to CAMKK2 - N-terminal
Tested applications
WB, ELISA, IHC-Pmore details
Cross reactivity
Reacts with
Human
Immunogen
Synthetic peptide from the N-terminal region of human CAMKK2 conjugated to KLH.
Positive control
293 cell lysates transiently transfected with the CAMKK2 gene. Human prostate carcinoma tissue.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.09% Sodium Azide
Constituents: PBS
Concentration
Concentration information loading...
Purity
Protein A purified
Purification notes
ab70908 is purified through a protein A column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Clonality
Polyclonal
Isotype
IgG
Applications
Show applications key
Our Abpromise guarantee covers the use of ab70908 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/50 - 1/100.Detects a band of approximately 72 kDa (predicted molecular weight: 65 kDa).
ELISA
ELISA: 1/1000ELISA: 1/1000
IHC-P: 1/10 - 1/50.
Target
Function
Calcium/calmodulin-dependent protein kinase belonging to a proposed calcium-triggered signaling cascade involved in a number of cellular processes. Isoform 1, isoform 2 and isoform 3 phosphorylate CAMK1 and CAMK4. Isoform 3 phosphorylates CAMK1D. Isoform 4, isoform 5 and isoform 6 lacking part of the calmodulin-binding domain are inactive. Seems to be involved in hippocampal activation of CREB1.
Tissue specificity
Ubiquitously expressed with higher levels in the brain. Intermediate levels are detected in spleen, prostate, thyroid and leukocytes. The lowest level is in lung.
Sequence similarities
Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.
Contains 1 protein kinase domain.
Domain
The autoinhibitory domain overlaps with the calmodulin binding region and may be involved in intrasteric autoinhibition.
The RP domain (arginine/proline-rich) is involved in the recognition of CAMKI and CAMK4 as substrates.
Post-translational
modifications
Autophosphorylated.
Cellular localization
Cytoplasm.
Target information above from: UniProt accessionQ96RR4
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Calcium/calmodulin dependent protein kinase beta antibody
- Calcium/calmodulin dependent protein kinase kinase 2 antibody
- Calcium/calmodulin dependent protein kinase kinase beta antibody
see all
Database links
- Entrez Gene: 10645 Human
- SwissProt: Q96RR4 Human
- Unigene: 297343 Human
Anti-CAMKK2 antibody - N-terminal images:
Western blot - CAMKK2 antibody (ab70908)
All lanes : Anti-CAMKK2 antibody - N-terminal (ab70908) at 1/50 dilution
Lane 1 : Nontransfected 293 cell lysate
Lane 2 : 293 cell lysate transiently transfected with the CAMKK2 gene
Lysates/proteins at 2 µg per lane.
Predicted band size : 65 kDa
Observed band size : 72 kDa (why is the actual band size different from the predicted?)
Additional bands at : 70 kDa. We are unsure as to the identity of these extra bands.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - CAMKK2 antibody - N-terminal (ab70908)
Formalin-fixed and paraffin-embedded human prostate carcinoma tissue labelled with ab70908 at 1/10 dilution followed by peroxidise conjugation to the secondary antibody and DAB staining.
References for Anti-CAMKK2 antibody - N-terminal (ab70908)
ab70908 has not yet been referenced specifically in any publications.
Publishing research using ab70908? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"