Recombinant Anti-CAP1 antibody [EPR8339(B)] (ab155079)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8339(B)] to CAP1
- Suitable for: Flow Cyt (Intra), WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-CAP1 antibody [EPR8339(B)]
See all CAP1 primary antibodies -
Description
Rabbit monoclonal [EPR8339(B)] to CAP1 -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), WB, IHC-Pmore details
Unsuitable for: ICC/IF or IP -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- HeLa, 293T, A431, HepG2 and Caco-2 cell lysates; 293 cells; Prostate tissue.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR8339(B) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab155079 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt (Intra) |
1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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WB | (4) |
1/1000 - 1/10000. Predicted molecular weight: 52 kDa.
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IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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Flow Cyt (Intra)
1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
WB
1/1000 - 1/10000. Predicted molecular weight: 52 kDa. |
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Directly regulates filament dynamics and has been implicated in a number of complex developmental and morphological processes, including mRNA localization and the establishment of cell polarity. -
Sequence similarities
Belongs to the CAP family.
Contains 1 C-CAP/cofactor C-like domain. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 10487 Human
- Entrez Gene: 12331 Mouse
- Entrez Gene: 64185 Rat
- Omim: 601896 Human
- SwissProt: Q01518 Human
- SwissProt: P40124 Mouse
- SwissProt: Q08163 Rat
- Unigene: 370581 Human
see all -
Alternative names
- Adenylyl cyclase associated protein 1 antibody
- Adenylyl cyclase-associated protein 1 antibody
- CAP 1 antibody
see all
Images
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All lanes : Anti-CAP1 antibody [EPR8339(B)] (ab155079) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : 293T cell lysate
Lane 3 : A431 cell lysate
Lane 4 : HepG2 cell lysate
Lane 5 : Caco-2 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 52 kDa -
Intracellular flow cytometric analysis of permeabilized 293 cells, labeling CAP1 with ab155079 at 1/10 dilution (red) or Rabiit IgG negative control (green).
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Immunohistochemical analysis of paraffin-embedded prostate tissue, labeling CAP1 with ab155079 at 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin embedded Human cervical carcinoma tissue using ab155079 showing +ve staining.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin embedded Human gastric adenocarcinoma tissue using ab155079 showing +ve staining.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin embedded Human normal liver tissue using ab155079 showing +ve staining.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin embedded Human lung adenocarcinoma tissue using ab155079 showing +ve staining.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (5)
ab155079 has been referenced in 5 publications.
- Hashimoto-Gotoh A et al. Persistent Infection of Simian Foamy Virus Derived from the Japanese Macaque Leads to the High-Level Expression of microRNA that Resembles the miR-1 microRNA Precursor Family. Microbes Environ 35:N/A (2020). PubMed: 31969530
- Liu Y et al. Adenylyl cyclase-associated protein 1-targeted nanoparticles as a novel strategy for the treatment of metastatic non-small cell lung cancer. Int J Oncol 55:462-472 (2019). PubMed: 31173184
- Sato H et al. Resistin upregulates chemokine production by fibroblast-like synoviocytes from patients with rheumatoid arthritis. Arthritis Res Ther 19:263 (2017). WB, IHC ; Human . PubMed: 29191223
- Krochmal M et al. Identification of novel molecular signatures of IgA nephropathy through an integrative -omics analysis. Sci Rep 7:9091 (2017). PubMed: 28831120
- Shao Z et al. LINGO-1 Regulates Oligodendrocyte Differentiation through the Cytoplasmic Gelsolin Signaling Pathway. J Neurosci 37:3127-3137 (2017). PubMed: 28193690