Anti-Carma 1/CARD11 antibody (ab91463)
Key features and details
- Rabbit polyclonal to Carma 1/CARD11
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Carma 1/CARD11 antibody
See all Carma 1/CARD11 primary antibodies -
Description
Rabbit polyclonal to Carma 1/CARD11 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Horse, Cow, Dog, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Platypus, Elephant -
Immunogen
Synthetic peptide corresponding to Human Carma 1/CARD11 aa 275-325.
Database link: NP_115791.3 -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab91463 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/2000 - 1/10000. Predicted molecular weight: 133 kDa.
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IP |
Use at 2-5 µg/mg of lysate.
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Notes |
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WB
1/2000 - 1/10000. Predicted molecular weight: 133 kDa. |
IP
Use at 2-5 µg/mg of lysate. |
Target
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Function
Involved in the costimulatory signal essential for T-cell receptor (TCR)-mediated T-cell activation. Its binding to DPP4 induces T-cell proliferation and NF-kappa-B activation in a T-cell receptor/CD3-dependent manner. Activates NF-kappa-B via BCL10 and IKK. Stimulates the phosphorylation of BCL10. -
Tissue specificity
Detected in adult peripheral blood leukocytes, thymus, spleen and liver. Also found in promyelocytic leukemia HL-60 cells, chronic myelogenous leukemia K562 cells, Burkitt's lymphoma Raji cells and colorectal adenocarcinoma SW480 cells. Not detected in HeLa S3, Molt-4, A549 and G431 cells. -
Sequence similarities
Contains 1 CARD domain.
Contains 1 guanylate kinase-like domain.
Contains 1 PDZ (DHR) domain. -
Post-translational
modificationsPhosphorylation at Ser-559, Ser-644 and Ser-652 by PRKCBB leads to a shift from an inactive to an active form that activates the NF-kappa-B signaling. -
Cellular localization
Cytoplasm. Membrane raft. Colocalized with DPP4 in membrane rafts. - Information by UniProt
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Database links
- Entrez Gene: 515377 Cow
- Entrez Gene: 84433 Human
- Entrez Gene: 108723 Mouse
- Entrez Gene: 304314 Rat
- Omim: 607210 Human
- SwissProt: Q9BXL7 Human
- SwissProt: Q8CIS0 Mouse
- Unigene: 648101 Human
see all -
Alternative names
- 0610008L17Rik antibody
- 2410011D02Rik antibody
- Bcl10 interacting maguk protein 3 antibody
see all
Images
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All lanes : Anti-Carma 1/CARD11 antibody (ab91463) at 0.1 µg/ml
Lane 1 : Ramos whole cell lysate at 50 µg
Lane 2 : Ramos whole cell lysate at 15 µg
Lane 3 : Ramos whole cell lysate at 5 µg
Lane 4 : K562 whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 133 kDa
Exposure time: 3 minutes -
Sample: whole cell lysate from Ramos cells (1 mg for IP, 20% of IP loaded) .
Antibody: ab91463 at 1 µg/ml for WB and at 10 µg/mg lysate for IP.
Detection: chemiluminescence with exposure times of 10 seconds.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab91463 has been referenced in 3 publications.
- Stinson JR et al. Gain-of-function mutations in CARD11 promote enhanced aggregation and idiosyncratic signalosome assembly. Cell Immunol 353:104129 (2020). PubMed: 32473470
- Zhu D et al. MicroRNA-181a Inhibits Activated B-Cell-Like Diffuse Large B-Cell Lymphoma Progression by Repressing CARD11. J Oncol 2019:9832956 (2019). PubMed: 31662757
- Snow AL et al. Congenital B cell lymphocytosis explained by novel germline CARD11 mutations. J Exp Med 209:2247-61 (2012). ICC/IF ; Human . PubMed: 23129749