Anti-CARD9 antibody (ab169623)
Key features and details
- Mouse polyclonal to CARD9
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-CARD9 antibody
See all CARD9 primary antibodies -
Description
Mouse polyclonal to CARD9 -
Host species
Mouse -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein within Human CARD9 aa 1-500. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
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Positive control
- CARD9 transfected 293T cell lysate; HeLa cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
pH: 7.4
Constituent: 99% PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab169623 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 1 µg/ml. Predicted molecular weight: 56 kDa.
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ICC/IF |
Use a concentration of 10 µg/ml.
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Notes |
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WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 56 kDa. |
ICC/IF
Use a concentration of 10 µg/ml. |
Target
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Function
Activates NF-kappa-B via BCL10. -
Tissue specificity
Highly expressed in spleen. Also detected in liver, placenta, lung, peripheral blood leukocytes and in brain. -
Involvement in disease
Defects in CARD9 are the cause of familial candidiasis type 2 (CANDF2) [MIM:212050]. Chronic mucocutaneous candidiasis is characterized by impaired clearance of fungal infections and results in colonization and infections of the mucosa or skin, predominantly with Candida albicans. CANDF2 is an autosomal recessive chronic mucocutaneous candidiasis. -
Sequence similarities
Contains 1 CARD domain. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 64170 Human
- Omim: 607212 Human
- SwissProt: Q9H257 Human
- Unigene: 694071 Human
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Alternative names
- CANDF2 antibody
- CARD9 antibody
- CARD9_HUMAN antibody
see all
Images
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All lanes : Anti-CARD9 antibody (ab169623) at 1 µg/ml
Lane 1 : CARD9 transfected 293T cell lysate
Lane 2 : Non-transfected 293T cell lysate
Lysates/proteins at 15 µl per lane.
Developed using the ECL technique.
Predicted band size: 56 kDa -
Immunofluorescent analysis of HeLa cells labeling CARD9 with ab169623 at 10 µg/ml.
Protocols
Datasheets and documents
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Datasheet download
References (1)
ab169623 has been referenced in 1 publication.
- Wang H et al. NOD-like receptors mediate inflammatory lung injury during plateau hypoxia exposure. J Physiol Anthropol 39:32 (2020). PubMed: 33028417