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Overview

  • Product nameCARHSP1 protein (Tagged-His Tag)See all CARHSP1 proteins and peptides ...
  • Protein descriptionRecombinant fragment, corresponding to amino acids 26-147 (122 amino acids) of Human CARHSP1 with N terminal His tag; Predicted MWt 14 kDa (UniProt Q9Y2V2).
  • Expression hostE. coli

    • Properties

  • Purification notesPurity level >85%
    This protein was expressed as an N-terminal His-tag fusion protein using Escherichia coli, and purified using Immobilized Metal Ion Affinity Chromatography. In some cases, smaller protein fragments may be present in addition to the intended expression product as a result of premature termination during translation in E. coli and subsequent co-purification via the His-tag. In some cases purified proteins run at a molecular weight different to the theoretically calculated molecular weight. This may be as a result of unequally distributed charges in the amino acid sequence. Alternatively, dimerisation of the expression product can occur under oxygen limitation during expression/cultivation.
  • FormLyophilised:Reconstitute with 73 µl aqua dest.
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 0.5% Trehalose, 6M Urea, 100mM Sodium phosphate, 10mM Sodium chloride, pH 4.5
  • Concentration information loading...
  • Additional notesProtein Identity confirmed by Mass Spectrometry (MS/MS) (acquired on initial reference batch).
  • Sequence notesSRERSPSPLRGNVVPSPLPTRRTRTFSATVRASQGPVYKG VCKCFCRSK GHGFITPADGGPDIFLHISDVEGEYVPVE GDEVTYKMCSIPPKNEKLQA VEVVITHLAPGTKHETWS GHVISS
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab92018 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    SDS-PAGE
    MS
  • Application notesMS: Use at an assay dependent dilution.
    SDS-PAGE: Use at an assay dependent dilution.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Protein info

    • Alternative names
        Calcium regulated heat stable protein (24kD)Calcium regulated heat stable protein 1Calcium regulated heat stable protein 1 (24kD)
        Calcium-regulated heat stable protein 1Calcium-regulated heat-stable protein of 24 kDaCarhsp1CHSP1_HUMANCRHSP-24CRHSP24CSDC1
      see all
  • FunctionBinds mRNA and regulates the stability of target mRNA. Binds single-stranded DNA (in vitro).
  • Sequence similaritiesContains 1 CSD (cold-shock) domain.
  • Post-translational
    modifications    Dephosphorylated by calcineurin in a Ca(2+) dependent manner.
  • Cellular localizationCytoplasm. Cytoplasm > P-body. Cytoplasmic granule. Detected at cytoplasmic stress granules and P-bodies. Detected at exosome granules where mRNA is degraded.

    • Target information above from: UniProt accession Q9Y2V2 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

    CARHSP1 protein (Tagged-His Tag) images

    • SDS-PAGE - CARHSP1 protein (Tagged-His Tag) (ab92018)
      SDS-PAGE - CARHSP1 protein (Tagged-His Tag) (ab92018)
      The image shows an electrophoretic assay performed using an Agilent 5100 ALP. In some images coloured control bands can be seen at 15 kDa (green) and/or 240 kDa (purple). The protein-specific band is blue.

    References for CARHSP1 protein (Tagged-His Tag) (ab92018)

    ab92018 has not yet been referenced specifically in any publications.

    Publishing research using ab92018 ? Please let us know so that we can cite the reference in this datasheet

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