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Western blot - CASK antibody (ab3383)
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Product Name
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CASK antibody
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See all CASK antibodies (2)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to CASK
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Immunogen
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Peptide corresponding to amino acid residues 578-592 from human CASK.
This sequence is completely conserved between rat, mouse, and human CASK.
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Reacts with
(species key)
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Hu, Ms, Rat
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Specificity
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By Western blot, this antibody detects a 112 kDa protein representing CASK from AtT20 cell lysate.
Immunohistochemical staining of CASK in mouse brain with this antibody results in staining of the choroid plexus.
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Epitope
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The epitope lies between amino acid residues 578-592 from human CASK.
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Tested applications
(see key)
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WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
WB: Use at an assay dependent dilution. Predicted molecular weight: 105 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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WB: AtT20 cell lysate
IHC: mouse brain
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Cellular localization
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Cytoplasmic (Potential).
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Research areas
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Neuroscience >> Cell Type Marker >> Neuron marker >> Synapse marker
Signal Transduction >> Signaling Pathway >> Calcium Signaling >> Calmodulin Pathway
Neuroscience >> Neurotransmission >> Intracellular Signaling >> Kinases
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Relevance
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Calmodulin sensitive kinase (CASK/mLIN-2) is a ~112-kDa member of the membrane associated quanylate kinase (MAGUK) protein family with multiple protein binding domains. These include an N-terminal calmodulin kinase (CaMKII) domain and C-terminal PDZ, SH3-, and quanylate kinase domains. CASK possesses both tyrosine kinase and serine/threonine kinase activity. In addition, CASK can form a heterotrimeric complex with Mint (X11or mLIN-10) and Veli (mLIN-7) proteins which is predicted to participate in plasma membrane receptor localization. CASK is localized to neuronal synapses and also interacts with syndecan and neurexin. The guanylate kinase domain of CASK has been shown to interact with the T-box transcription factor, Tbr-1, as a coactivator to induce transcription of genes that contain T-elements.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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PBS, 1 mg/ml BSA, 0.05% sodium azide Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this CASK antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for CASK antibody images, references, products related to ab3383 and other tools.
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CASK antibody images:
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 Western blot - CASK antibody (ab3383)
Predicted band size : 105 kDa
ab3383 at a 1µg/ml concentration staining CASK in AtT20 cell lysate by Western blot.
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Search PubMed (MEDLINE) for references to CASK
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CASK antibody - more information
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Customer reviews (feedback) regarding CASK antibody |
Customer FAQs regarding CASK antibody |
Protocols for CASK antibody |
Price and availability of products related to CASK antibody |
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CASK antibody - related products:
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CASK antibody - other tools:
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Contact Abcam with a Technical Enquiry about CASK antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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