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Products:Neuroscience >> Neurotransmission >> Intracellular Signaling >> Kinases
Overview
Product name
Anti-CASK antibody
See all CASK products (5) ...
Description
Rabbit polyclonal to CASK
Specificity
By Western blot, this antibody detects a 112 kDa protein representing CASK from AtT20 cell lysate. Immunohistochemical staining of CASK in mouse brain with this antibody results in staining of the choroid plexus.
Tested applications
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
Peptide corresponding to amino acid residues 578-592 from human CASK. This sequence is completely conserved between rat, mouse, and human CASK.
Epitope
The epitope lies between amino acid residues 578-592 from human CASK.
Positive control
WB: AtT20 cell lysate IHC: mouse brain
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
PBS, 1 mg/ml BSA, 0.05% sodium azide
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Neuroscience >> Cell Type Marker >> Neuron marker >> Synapse marker
Signal Transduction >> Signaling Pathway >> Calcium Signaling >> Calmodulin Pathway
Neuroscience >> Neurotransmission >> Intracellular Signaling >> Kinases
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Western blot - CASK antibody (ab3383)
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab3383 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use at an assay dependent dilution. Predicted molecular weight: 105 kDa.
Target
Function
Multidomain scaffolding protein with a role in synaptic transmembrane protein anchoring and ion channel trafficking. Contributes to neural development and regulation of gene expression via interaction with the transcription factor TRB1. Binds to cell-surface proteins, including amyloid precursor protein, neurexins and syndecans. May mediate a link between the extracellular matrix and the actin cytoskeleton via its interaction with syndecan and with the actin/spectrin-binding protein 4.1.
Tissue specificity
Ubiquitous. Expression is significantly greater in brain relative to kidney, lung, and liver and in fetal brain and kidney relative to lung and liver.
Involvement in disease
Defects in CASK are the cause of mental retardation X-linked CASK-related (MRXCASK) [MIM:300749]. Mental retardation is characterized by significantly below average general intellectual functioning associated with impairments in adaptative behavior and manifested during the developmental period. Patients with mental retardation X-linked CASK-related can manifest a severe phenotype consisting of severe intellectual deficit, congenital or postnatal microcephaly, disproportionate brainstem and cerebellar hypoplasia (MICPCH Syndrome). A milder phenotype consists of mental retardation alone or associated with nystagmus.
Defects in CASK are the cause of FG syndrome type 4 (FGS4) [MIM:300422]. FG syndrome (FGS) is an X-linked disorder characterized by mental retardation, relative macrocephaly, hypotonia and constipation.
Sequence similarities
In the N-terminal section; belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily.
Belongs to the MAGUK family.
Contains 1 guanylate kinase-like domain.
Contains 2 L27 domains.
Contains 1 PDZ (DHR) domain.
Contains 1 protein kinase domain.
Contains 1 SH3 domain.
Domain
The first L27 domain binds DLG1 and the second L27 domain probably binds LIN7.
The protein kinase domain mediates the interaction with FCHSD2.
Cellular localization
Nucleus. Cytoplasm. Cell membrane.
Target information above from: UniProt accessionO14936
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- CAGH39 antibody
- Calcium/calmodulin dependent serine protein kinase antibody
- Calcium/calmodulin dependent serine protein kinase (MAGUK family) antibody
see all
Database links
- Entrez Gene: 8573 Human
- Entrez Gene: 12361 Mouse
- Entrez Gene: 29647 Rat
- Omim: 300172 Human
- SwissProt: O14936 Human
- SwissProt: O70589 Mouse
- SwissProt: Q62915 Rat
- Unigene: 495984 Human
see all
Anti-CASK antibody images:
Western blot - CASK antibody (ab3383)
Predicted band size : 105 kDa
ab3383 at a 1µg/ml concentration staining CASK in AtT20 cell lysate by Western blot.
References for Anti-CASK antibody (ab3383)
This product has been referenced in:
- Ojeh Net al. The MAGUK-family protein CASK is targeted to nuclei of the basal epidermis and controls keratinocyte proliferation. J Cell Sci 121:2705-17 (2008). IHC; Mouse, Rat.Read more (PubMed: 18664494) »
See 1 publication for this product
Publishing research using ab3383? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"