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Proteins and Peptides
ab37587
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Secondary antibodies
Reassurance, Refunds & Replacements
If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »Publishing research using ab37588? Please let us know so that we can cite the reference in this datasheet
ab37588 has been referenced in 1 publications.
- Barilli A et al. Arginine transport in human monocytic leukemia THP-1 cells during macrophage differentiation. J Leukoc Biol : (2011). WB; Human. PubMed: 21586674
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Western blot - CAT1 antibody (ab37588)
All lanes : Anti-CAT1 antibody (ab37588) at 1 µg/ml
Lane 1 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 2 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lane 3 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size : 68 kDa
Observed band size : 68 kDa
Immunocytochemistry/ Immunofluorescence - CAT1 antibody (ab37588)
ICC/IF image of ab37588 stained human HeLa cells. The cells were PFA fixed (10 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab37588, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
Immunohistochemistry (PFA perfusion fixed frozen sections) - CAT1 antibody (ab37588)
IHC-FoFr image of CAT1 staining on Mouse Spinal Cord sections using ab37588 (1:1000). The sections used came from animals perfused fixed with Paraformaldehyde 4% with 15% of a solution of saturated picric acid, in phosphate buffer 0.1M. Following postfixation in the same fixative overnight, the spinal cord were cryoprotected in sucrose 30% overnight. Spinal cords were then cut using a cryostat and the immunostainings were performed using the ‘free floating’ technique.
Sophie Pezet, ESPCI, France
CAT1 antibody for Immunohistochemistry (PFA perfusion fixed frozen sections) in Mouse (37588)
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