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Immunocytochemistry/ Immunofluorescence - CAT1 antibody (ab37588)
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Western blot - CAT1 antibody (ab37588)
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - CAT1 antibody (ab37588)
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Product Name
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CAT1 antibody
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See all CAT1 antibodies (2)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to CAT1
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Immunogen
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Synthetic peptide conjugated to KLH derived from within residues 600 to the C-terminus of Human CAT1 (gene: SLC7A1). (Peptide available as ab37587.)
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Reacts with
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Hu
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Predicted to react
(species key)
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Orngt
(Due to sequence homology)
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Tested applications
(see key)
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ICC/IF, IHC-P, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ICC/IF: Use at a concentration of 1 µg/ml.
IHC-P: Use at a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB: 1/250. Detects a band of approximately 68 kDa (predicted molecular weight: 68 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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This antibody gave a positive signal in the following human whole cell lysates: Jurkat, MCF-7 and HepG2.
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Cellular localization
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Integral membrane protein.
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Research areas
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Signal Transduction >> Metabolism >> Plasma Membrane >> Channels
Neuroscience >> Neurotransmission >> Receptors / Channels >> More Ion Channels
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Relevance
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CAT1 is a high-affinity, low capacity permease involved in the transport of the cationic amino acids (arginine, lysine and ornithine) in non-hepatic tissues. It may also function as an ecotropic retroviral leukemia receptor. It belongs to the amino acid-polyamine-organocation (APC) superfamily.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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0.100 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this CAT1 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for CAT1 antibody images, references, products related to ab37588 and other tools.
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CAT1 antibody images:
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 Immunocytochemistry/ Immunofluorescence - CAT1 antibody (ab37588)
ICC/IF image of ab37588 stained human HeLa cells. The cells were PFA fixed (10 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab37588, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
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Western blot - CAT1 antibody (ab37588)
All lanes : CAT1 antibody (ab37588) at 1 µg/ml
Lane 1 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 2 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lane 3 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size : 68 kDa
Observed band size : 68 kDa
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - CAT1 antibody (ab37588)
IHC image of CAT1 staining in FFPE human breast carcinoma FFPE section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab37588, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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Search PubMed (MEDLINE) for references to CAT1
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CAT1 antibody - more information
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Customer reviews (feedback) regarding CAT1 antibody |
Customer FAQs regarding CAT1 antibody |
Protocols for CAT1 antibody |
Price and availability of products related to CAT1 antibody |
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CAT1 antibody - related products:
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CAT1 antibody - other tools:
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Contact Abcam with a Technical Enquiry about CAT1 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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