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Anti-CAT1 antibody (ab37588)

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Overview

Product name

Anti-CAT1 antibody
See all CAT1 products (2) ...

Description

Rabbit polyclonal to CAT1

Tested applications

IHC-FoFr, ICC/IF, WBmore details

Cross reactivity

Reacts with

Mouse, Human

Predicted to work with

Orangutan

Immunogen

Synthetic peptide conjugated to KLH derived from within residues 600 to the C-terminus of Human CAT1 (gene: SLC7A1).

(Peptide available as ab37587.)

Positive control

This antibody gave a positive signal in the following human whole cell lysates: Jurkat, MCF-7 and HepG2.

Properties

Form

Liquid

Storage instructions

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4

Concentration

Concentration information loading...

Purity

Immunogen affinity purified

Clonality

Polyclonal

Isotype

IgG

  • Western blot - CAT1 antibody (ab37588)Western blot - CAT1 antibody (ab37588) image (enlarge)

  • Immunocytochemistry/ Immunofluorescence - CAT1 antibody (ab37588)Immunocytochemistry/ Immunofluorescence - CAT1 antibody (ab37588) image (enlarge)

  • Immunohistochemistry (PFA perfusion fixed frozen sections) - CAT1 antibody (ab37588)Immunohistochemistry (PFA perfusion fixed frozen sections) - CAT1 antibody (ab37588) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab37588 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Target

Function

High-affinity, low capacity permease involved in the transport of the cationic amino acids (arginine, lysine and ornithine) in non-hepatic tissues. May also function as an ecotropic retroviral leukemia receptor.

Tissue specificity

Ubiquitous.

Sequence similarities

Belongs to the amino acid-polyamine-organocation (APC) superfamily. Cationic amino acid transporter (CAT) (TC 2.A.3.3) family.

Cellular localization

Membrane.

Target information above from: UniProt accessionP30825 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • Amino acid transporter cationic 1 antibody
  • ATRC1 antibody
  • CAT-1 antibody
  • CaT1 antibody
  • CTR1_HUMAN antibody
  • Ecotropic retroviral leukemia receptor homolog antibody
  • Ecotropic retroviral receptor antibody
  • Ecotropic retrovirus receptor homolog antibody
  • ERR antibody
  • HCAT1 antibody
  • High affinity cationic amino acid transporter 1 antibody
  • High-affinity cationic amino acid transporter-1 antibody
  • REC1L antibody
  • SLC7A1 antibody
  • Solute carrier family 7 (cationic amino acid transporter, y+ system) member 1 antibody
  • Solute carrier family 7 member 1 antibody
  • System Y+ basic amino acid transporter antibody
see all

Anti-CAT1 antibody images:

  Western blot - CAT1 antibody (ab37588)

Western blot - CAT1 antibody (ab37588)

All lanes : Anti-CAT1 antibody (ab37588) at 1 µg/ml

Lane 1 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 2 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lane 3 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate

Lysates/proteins at 20 µg per lane.

Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

Performed under reducing conditions.

Predicted band size : 68 kDa
Observed band size : 68 kDa

  Immunocytochemistry/ Immunofluorescence - CAT1 antibody (ab37588)

Immunocytochemistry/ Immunofluorescence - CAT1 antibody (ab37588)

ICC/IF image of ab37588 stained human HeLa cells. The cells were PFA fixed (10 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab37588, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).

  Immunohistochemistry (PFA perfusion fixed frozen sections) - CAT1 antibody (ab37588)

Immunohistochemistry (PFA perfusion fixed frozen sections) - CAT1 antibody (ab37588)

IHC-FoFr image of CAT1 staining on Mouse Spinal Cord sections using ab37588 (1:1000). The sections used came from animals perfused fixed with Paraformaldehyde 4% with 15% of a solution of saturated picric acid, in phosphate buffer 0.1M. Following postfixation in the same fixative overnight, the spinal cord were cryoprotected in sucrose 30% overnight. Spinal cords were then cut using a cryostat and the immunostainings were performed using the ‘free floating’ technique.

Sophie Pezet, ESPCI, France

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References for Anti-CAT1 antibody (ab37588)

This product has been referenced in:

  • Barilli Aet al. Arginine transport in human monocytic leukemia THP-1 cells during macrophage differentiation. J Leukoc Biol : (2011). WB; Human.Read more (PubMed: 21586674) »

See 1 publication for this product

Publishing research using ab37588? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"