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Immunocytochemistry/ Immunofluorescence - CAT1 antibody (ab37588)
Immunocytochemistry/ Immunofluorescence - CAT1 antibody (ab37588)
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Western blot - CAT1 antibody (ab37588)
Western blot - CAT1 antibody (ab37588)
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - CAT1 antibody (ab37588)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - CAT1 antibody (ab37588)
(enlarge)


Product Name 

CAT1 antibody

 

See all CAT1 antibodies (2)...

Product type 

Primary antibodies

Description 

Rabbit polyclonal to CAT1

Immunogen 

Synthetic peptide conjugated to KLH derived from within residues 600 to the C-terminus of Human CAT1 (gene: SLC7A1).

(Peptide available as ab37587.)

Reacts with 

Hu

Predicted to react 
(species key)

Orngt
(Due to sequence homology)

Tested applications 
(see key)

ICC/IF, IHC-P, WB


Abreviews 

Customer reviews
(see key)


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Application notes 
(see key)

Recommended dilutions
ICC/IF: Use at a concentration of 1 µg/ml.
IHC-P: Use at a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB: 1/250. Detects a band of approximately 68 kDa (predicted molecular weight: 68 kDa).


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

This antibody gave a positive signal in the following human whole cell lysates: Jurkat, MCF-7 and HepG2.

Cellular localization 

Integral membrane protein.

Research areas 

Signal Transduction >> Metabolism >> Plasma Membrane >> Channels
Neuroscience >> Neurotransmission >> Receptors / Channels >> More Ion Channels

Relevance 

CAT1 is a high-affinity, low capacity permease involved in the transport of the cationic amino acids (arginine, lysine and ornithine) in non-hepatic tissues. It may also function as an ecotropic retroviral leukemia receptor. It belongs to the amino acid-polyamine-organocation (APC) superfamily.

 

Alternative names CAT1 antibody (ab37588)

Database links 

The links below go to external sites and will open in a new browser window

Entrez Gene

    

6541    (Human)

Omim

    

104615    (Human)

SwissProt

    

P30825    (Human)
Q96D65    (Human)
Q5JR50    (Human)
Q5JR52    (Human)

Unigene

    

14846    (Human)

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Concentration 

0.100 mg/ml

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

 

At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this CAT1 antibody is on this datasheet. But please do contact us if you would like any reassurance!


See below for CAT1 antibody images, references, products related to ab37588 and other tools.

 

CAT1 antibody images:

Product image  Immunocytochemistry/ Immunofluorescence - CAT1 antibody (ab37588)

Immunocytochemistry/ Immunofluorescence - CAT1 antibody (ab37588)

ICC/IF image of ab37588 stained human HeLa cells. The cells were PFA fixed (10 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab37588, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).

 

Product image  Western blot - CAT1 antibody (ab37588)

Western blot - CAT1 antibody (ab37588)

All lanes : CAT1 antibody (ab37588) at 1 µg/ml

Lane 1 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 2 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lane 3 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate

Lysates/proteins at 20 µg per lane.

Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

Performed under reducing conditions.

Predicted band size : 68 kDa
Observed band size : 68 kDa

 

Product image  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - CAT1 antibody (ab37588)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - CAT1 antibody (ab37588)

IHC image of CAT1 staining in FFPE human breast carcinoma FFPE section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab37588, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

 
 

Search PubMed (MEDLINE) for references to CAT1

 

CAT1 antibody - more information

Customer reviews (feedback) regarding CAT1 antibody

Customer FAQs regarding CAT1 antibody

Protocols for CAT1 antibody

Price and availability of products related to CAT1 antibody

 

CAT1 antibody - related products:

Products for Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Slides

Products for Western blot

Loading controls

Lysates

 

Immunizing Peptide for CAT1 antibody

ab37587

  

CAT1 peptide

 

Compatible Secondaries for CAT1 antibody

ab6564

  

Cy5 ® antibody

ab6717

  

FITC antibody

ab6720

  

Biotin antibody

ab6721

  

HRP antibody

ab6722

  

Alkaline Phosphatase antibody

ab6793

  

Texas Red ® antibody

ab7007

  

Phycoerythrin antibody

ab7019

  

Agarose antibody

ab27234

  

10nm Gold antibody

ab27235

  

5nm Gold antibody

ab27236

  

15nm Gold antibody

ab27237

  

20nm Gold antibody

ab27238

  

30nm Gold antibody

ab27239

  

10nm Gold antibody

ab27240

  

5nm Gold antibody

ab31193

  

Duck polyclonal to Rabbit IgG

ab60314

  

Chromeo™ 488 antibody

ab60315

  

Chromeo™ 494 antibody

ab60317

  

Chromeo™ 546 antibody

ab60319

  

Chromeo™ 642 antibody

 

Other CAT1 Antibodies

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CAT1 antibody - other tools:

Contact Abcam with a Technical Enquiry about CAT1 antibody

 

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