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Anti-CBF1 interacting corepressor antibody (ab50830)

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Overview

Product name

Anti-CBF1 interacting corepressor antibody
See all CBF1 interacting corepressor products (6) ...

Description

Rabbit polyclonal to CBF1 interacting corepressor

Tested applications

ELISA, WBmore details

Cross reactivity

Reacts with

Human

Immunogen

Synthetic peptide (Human)

Properties

Form

Lyophilised:
Add 50 µl of distilled water.

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: None
Constituents: 2% Sucrose, PBS

Concentration

Concentration information loading...

Purity

Immunogen affinity purified

Clonality

Polyclonal

Isotype

IgG

Research areas

Epigenetics and Nuclear Signaling >> Transcription >> Transcription Factors
Epigenetics and Nuclear Signaling >> Transcription >> Other factors
Epigenetics and Nuclear Signaling >> DNA / RNA >> RNA Processing >> Splicing

  • Western blot - CBF1 interacting corepressor antibody (ab50830)Western blot - CBF1 interacting corepressor antibody (ab50830) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab50830 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application notes

ELISA: Use at an assay dependent dilution. Titre using peptide based assay: 1:62500
WB: Use at a concentration of 0.25 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 52 kDa).

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Target

Relevance

CBF1 interacting corepressor may modulate splice site selection during alternative splicing of pre-mRNAs. It regulates transcription and acts as corepressor for RBPSUH; recruits RBPSUH to the Sin3-histone deacetylase complex (HDAC).

Cellular localization

Nuclear

Alternative names

  • CIR antibody
  • Recepin antibody

Database links

Anti-CBF1 interacting corepressor antibody images:

  Western blot - CBF1 interacting corepressor antibody (ab50830)

Western blot - CBF1 interacting corepressor antibody (ab50830)

All lanes : Anti-CBF1 interacting corepressor antibody (ab50830) at 0.25 µg/ml

Lane 1 : MW ladder
Lane 2 : Transfected 293T cell lysate

Secondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilution

Predicted band size : 52 kDa
Observed band size : 60 kDa (why is the actual band size different from the predicted?)
Additional bands at : 36 kDa,40 kDa,75 kDa. We are unsure as to the identity of these extra bands.

References for Anti-CBF1 interacting corepressor antibody (ab50830)

ab50830 has not yet been referenced specifically in any publications.

Publishing research using ab50830? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"