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Western blot - CBF1 interacting corepressor antibody (ab50830)
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Product Name
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CBF1 interacting corepressor antibody
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See all CBF1 interacting corepressor antibodies (5)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to CBF1 interacting corepressor
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Immunogen
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Synthetic peptide (Human)
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Reacts with
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Hu
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Tested applications
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ELISA, WB
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Abreviews
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Application notes
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Recommended dilutions
ELISA: Use at an assay dependent dilution. Titre using peptide based assay: 1:62500
WB: Use at a concentration of 0.25 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 52 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Cellular localization
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Nuclear
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Research areas
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Chromatin and Nuclear Signaling >> Transcription >> Transcription Factors
Chromatin and Nuclear Signaling >> Transcription >> Other factors
Chromatin and Nuclear Signaling >> DNA / RNA >> RNA Processing >> Splicing
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Relevance
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CBF1 interacting corepressor may modulate splice site selection during alternative splicing of pre-mRNAs. It regulates transcription and acts as corepressor for RBPSUH; recruits RBPSUH to the Sin3-histone deacetylase complex (HDAC).
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: None
Constituents: 2% Sucrose, PBS
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Form
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Lyophilised
Add 50 µl of distilled water.
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Concentration
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1.000 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this CBF1 interacting corepressor antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for CBF1 interacting corepressor antibody images, references, products related to ab50830 and other tools.
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CBF1 interacting corepressor antibody images:
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 Western blot - CBF1 interacting corepressor antibody (ab50830)
All lanes : CBF1 interacting corepressor antibody (ab50830) at 0.25 µg/ml
Lane 1 : MW ladder
Lane 2 : Transfected 293T cell lysate
Secondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size : 52 kDa
Observed band size : 60 kDa (why is the actual band size different from the predicted?)
Additional bands at : 36 kDa,40 kDa,75 kDa. We are unsure as to the identity of these extra bands.
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Search PubMed (MEDLINE) for references to CBF1 interacting corepressor
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CBF1 interacting corepressor antibody - more information
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CBF1 interacting corepressor antibody - related products:
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CBF1 interacting corepressor antibody - other tools:
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Contact Abcam with a Technical Enquiry about CBF1 interacting corepressor antibody |
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