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CBL 293T Transfected Lysate - (positive control) (ab94206)

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Overview

  • Product nameCBL 293T Transfected Lysate - (positive control)
  • General notesab94206 is a 293T cell transfected lysate in which Human CBL has been transiently over-expressed using a pCMV-CBL plasmid. The lysate is provided in 1 x Sample Buffer. Note: For more details about how the transfected lysate was prepared view preparation notes
  • Tested applicationsWB more details

Properties

  • BackgroundFunction: Participates in signal transduction in hematopoietic cells. Adapter protein that functions as a negative regulator of many signaling pathways that start from receptors at the cell surface. Acts as an E3 ubiquitin-protein ligase, which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes, and then transfers it to substrates promoting their degradation by the proteasome. Recognizes activated receptor tyrosine kinases, including PDGFA, EGF and CSF1, and terminates signaling. Pathway: Protein modification; protein ubiquitination. Disease: Defects in CBL are the cause of Noonan syndrome-like disorder (NSL) [MIM:613563]. NSL is a syndrome characterized by a phenotype reminiscent of Noonan syndrome. Clinical features are highly variable, including facial dysmorphism, short neck, developmental delay, hyperextensible joints and thorax abnormalities with widely spaced nipples. The facial features consist of triangular face with hypertelorism, large low-set ears, ptosis, and flat nasal bridge. Some patients manifest cardiac defects. Similarity: Contains 1 Cbl-PTB (Cbl-type phosphotyrosine-binding) domain. Contains 1 RING-type zinc finger. Contains 1 UBA domain. Domain: The RING-type zinc finger domain mediates binding to an E2 ubiquitin-conjugating enzyme. The N-terminus is composed of the phosphotyrosine binding (PTB) domain, a short linker region and the RING-type zinc finger. The PTB domain, which is also called TKB (tyrosine kinase binding) domain, is composed of three different subdomains: a four-helix bundle (4H), a calcium-binding EF hand and a divergent SH2 domain. PTM: Phosphorylated on tyrosine residues by EGFR, SYK, FYN and ZAP70 (By similarity). Phosphorylated on tyrosine residues by INSR.

    • Applications

    Our Abpromise guarantee covers the use of ab94206 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    WB
  • Application notesWB: Use at an assay dependent dilution.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • CBL 293T Transfected Lysate - (positive control) images

    • SDS-PAGE - CBL 293T Transfected Lysate - (positive control) (ab94206)
      SDS-PAGE - CBL 293T Transfected Lysate - (positive control) (ab94206)
      ab94206 at 15µg/lane on an SDS-PAGE gel.
    • Western blot - CBL 293T Transfected Lysate - (positive control) (ab94206)
      Western blot - CBL 293T Transfected Lysate - (positive control) (ab94206)
      All lanes : Anti-CBL antibody (ab89529) at 1/500 dilution

      Lane 1 : CBL 293T Transfected Lysate - (positive control) (ab94206)
      Lane 2 : 293T non-transfected lysate

      Lysates/proteins at 25 µg per lane.

      Secondary
      Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution

    References for CBL 293T Transfected Lysate - (positive control) (ab94206)

    ab94206 has not yet been referenced specifically in any publications.

    Publishing research using ab94206 ? Please let us know so that we can cite the reference in this datasheet

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"