|
|
|
|
Western blot - CBL antibody (ab2235)
(enlarge)
|
|
|
|
|
Product Name
|
CBL antibody
|
| |
See all CBL antibodies (16)...
|
|
Product type
|
Primary antibodies
|
|
Description
|
Goat polyclonal to CBL
|
|
Immunogen
|
Synthetic peptide: REFVSISSPAHVAT, corresponding to C terminal amino acids 893-906 of Human CBL.
BLAST 'C-REFVSISSPAHVAT' with  or 
|
|
Reacts with
|
Ms
|
|
Predicted to react
(species key)
|
Hu, Rat
(Due to sequence homology)
|
|
Tested applications
(see key)
|
WB
|
|
|
Abreviews
|
|
|
|
Application notes
(see key)
|
Recommended dilutions
WB: Use at a concentration of 1 - 3 µg/ml. Detects a band of approximately 120 kDa in 3T3 lysate.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
|
|
Cellular localization
|
Cytoplasmic
|
|
Research areas
|
Cancer >> Oncoproteins/suppressors >> Oncoproteins >> Other
Cancer >> Signal transduction >> Nuclear signaling >> Nuclear hormone receptors >> Coactivators and repressors
Chromatin and Nuclear Signaling >> Nuclear Signaling Pathways >> Nuclear Receptors >> Co-activators/co-repressors
Chromatin and Nuclear Signaling >> Transcription >> Cancer susceptibility >> Proto-oncogenes
Signal Transduction >> Signaling Pathway >> Nuclear Signaling >> Nuclear Hormone Receptors >> Co-activators/co-repressors
Signal Transduction >> Adapters >> Cytoplasmic
Cell Biology >> Proteolysis / Ubiquitin >> Proteasome / Ubiquitin >> Ubiquitin E2s and E3s >> RING Finger E3 Ligase
|
|
Relevance
|
The proto-oncogene c-CBL was initially identified as the cellular homologue of v-CBL oncogene that induces pre-B cell lymphomas and myeloid leukemias in mice. In more recent studies CBL has been shown to be a negative regulator of tyrosine kinase signaling. The ubiquitin ligase activity of CBL leads to the degradation of tyrosine kinases, thus attenuating the signal of receptors. Targets of CBL include activated protein tyrosine kinases belonging to the Src and Syk/Zap-70 families. An additional mechanism to attenuate receptor signaling is thought to be achieved by CBL’s interaction with downstream targets of tyrosine kinases, such as PI-3K and Vav.
|
|
|
|
|
Database links
|
The links below go to external sites and will open in a new browser window |
|
Raised in
|
Goat
|
|
Clonality
|
Polyclonal
|
|
Isotype
|
IgG
|
|
Purity
|
IgG fraction
|
|
Storage buffer
|
Tris saline, 0.02% Na azide, pH7.3 with 0.5% BSA Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
|
|
Purification notes
|
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
|
|
Form
|
Liquid
|
|
Concentration
|
0.500 mg/ml
|
|
Storage instructions
|
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
|
| |
At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this CBL antibody is on this datasheet. But please do contact us if you would like any reassurance! |
 |
|
|
See below for CBL antibody images, references, products related to ab2235 and other tools.
|
| |
CBL antibody images:
|
 Western blot - CBL antibody (ab2235)
Ab2235 staining (2µg/ml) of 3T3 lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence
|
| |
| |
|
Search PubMed (MEDLINE) for references to CBL
|
| |
CBL antibody - more information
|
Customer reviews (feedback) regarding CBL antibody |
Customer FAQs regarding CBL antibody |
Protocols for CBL antibody |
Price and availability of products related to CBL antibody |
| |
CBL antibody - related products:
|
|
|
| |
CBL antibody - other tools:
|
Contact Abcam with a Technical Enquiry about CBL antibody |
| |
All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Pricing information loading
