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Anti-CBL antibody (ab6077)

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1 question for ab6077

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Question 1

Thursday 25-May-2006

thank you for the quick response and suggestions regarding the Cbl antibody (ab6077). I think that there may be the possibility that ab6077 recognizes (or was raised against an epitope of) human Cbl-B and not c-Cbl. Cbl-B has the Swiss Prot entry: Q13191 and encodes a 982 aa protein (=109.45 kDa) that has the identical N-terminus as c-Cbl. Ab6077 is raised against the C-terminus according to the reference data sheet and clearly recognizes a ~120 kDa band in our hands and also according to the data sheet. I think this band is more likely to correspond to Cbl-B (109 kDa) than to c-Cbl (Swiss prot entry P22681, ~99 kDa). Thank you for your suggestion regarding how to do our Western-Blots. I can assure you (and have attached a jpeg file of the Blot), that we do not get significant background, even with 70 ug of total protein loaded and that the only band we detect is the above mentioned 120 kDa band. I kindly ask you to find out about the sequence of the peptide/recombinant protein that was used for immunization to create ab6077. This will tell us without any doubts whether ab6077 is a c-Cbl or a Cbl-B antibody. In the meantime, I would appreciate if you could send us a complimentary aliquot of ab2235 so that we can test if we detect the same band as with ab6077.

ANSWER:

  

Thank you for your enquiry.

In Charlotte's absence I am dealing with her enquiries. She has been in touch with the source of this antibody and I can tell you that ab6077 was raised against a synthetic peptide: REFVSISSPAHVAT. Furthermore c-Cbl has been reported to run at p120, even though its theoretical MW is 99 kDa. In the literature it is referenced as proto-oncogene c-Cbl p120.

I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

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