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Products:Cell Biology >> Proteolysis / Ubiquitin >> Proteasome / Ubiquitin >> Ubiquitin E2s and E3s >> RING Finger E3 Ligase
Overview
Product name
Anti-CBL antibody
See all CBL products (19) ...
Description
Rabbit polyclonal to CBL
Tested applications
IHC-P, WB, IP, IHC-Frmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
Synthetic peptide: REFVSISSPAHVAT mapping to the carboxy terminal domain of human CBL.
REFVSISSPA HVAT
Properties
Form
Liquid
Storage instructions
Store at +4°C. Do not freeze.
Storage buffer
Preservative: 0.1% Sodium Azide
Constituents: 0.2% Gelatin, PBS
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Oncoproteins/suppressors >> Oncoproteins >> Other
Cancer >> Signal transduction >> Nuclear signaling >> Nuclear hormone receptors >> Coactivators and repressors
Epigenetics and Nuclear Signaling >> Nuclear Signaling Pathways >> Nuclear Receptors >> Co-activators/co-repressors
Epigenetics and Nuclear Signaling >> Transcription >> Cancer susceptibility >> Proto-oncogenes
Signal Transduction >> Signaling Pathway >> Nuclear Signaling >> Nuclear Hormone Receptors >> Co-activators/co-repressors
Signal Transduction >> Adapters >> Cytoplasmic
Cell Biology >> Proteolysis / Ubiquitin >> Proteasome / Ubiquitin >> Ubiquitin E2s and E3s >> RING Finger E3 Ligase
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-CBL antibody(ab6077)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab6077 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: Use at an assay dependent dilution.
WB: Use at an assay dependent dilution. Predicted molecular weight: 109 kDa.
IP: Use at an assay dependent dilution.
IHC-Fr: Use at an assay dependent dilution.
Target
Function
Participates in signal transduction in hematopoietic cells. Adapter protein that functions as a negative regulator of many signaling pathways that start from receptors at the cell surface. Acts as an E3 ubiquitin-protein ligase, which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes, and then transfers it to substrates promoting their degradation by the proteasome. Recognizes activated receptor tyrosine kinases, including PDGFA, EGF and CSF1, and terminates signaling.
Pathway
Protein modification; protein ubiquitination.
Involvement in disease
Defects in CBL are the cause of Noonan syndrome-like disorder (NSL) [MIM:613563]. NSL is a syndrome characterized by a phenotype reminiscent of Noonan syndrome. Clinical features are highly variable, including facial dysmorphism, short neck, developmental delay, hyperextensible joints and thorax abnormalities with widely spaced nipples. The facial features consist of triangular face with hypertelorism, large low-set ears, ptosis, and flat nasal bridge. Some patients manifest cardiac defects.
Sequence similarities
Contains 1 Cbl-PTB (Cbl-type phosphotyrosine-binding) domain.
Contains 1 RING-type zinc finger.
Contains 1 UBA domain.
Domain
The RING-type zinc finger domain mediates binding to an E2 ubiquitin-conjugating enzyme.
The N-terminus is composed of the phosphotyrosine binding (PTB) domain, a short linker region and the RING-type zinc finger. The PTB domain, which is also called TKB (tyrosine kinase binding) domain, is composed of three different subdomains: a four-helix bundle (4H), a calcium-binding EF hand and a divergent SH2 domain.
Post-translational
modifications
Phosphorylated on tyrosine residues by EGFR, SYK, FYN and ZAP70 (By similarity). Phosphorylated on tyrosine residues by INSR.
Cellular localization
Cytoplasm.
Target information above from: UniProt accessionP22681
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- 4732447J05Rik antibody
- C CBL antibody
- Cas Br M (murine) ecotropic retroviral transforming sequence antibody
see all
Database links
- Entrez Gene: 867 Human
- Entrez Gene: 12402 Mouse
- Entrez Gene: 500985 Rat
- Omim: 165360 Human
- SwissProt: P22681 Human
- SwissProt: P22682 Mouse
- Unigene: 504096 Human
- Unigene: 266871 Mouse
see all
Anti-CBL antibody images:
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-CBL antibody(ab6077)
Ab6077 staining human normal colon tissue. Staining is localised to cytoplasm.
Left panel: with primary antibody at 2 ug/ml. Right panel: isotype control.
Sections were stained using an automated system (DAKO Autostainer Plus ), at room temperature: sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
References for Anti-CBL antibody (ab6077)
ab6077 has not yet been referenced specifically in any publications.
Publishing research using ab6077? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"