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KAT3A / CBP protein (Tagged) (ab56272)

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Overview

  • Product nameKAT3A / CBP protein (Tagged)See all KAT3A / CBP proteins and peptides ...
  • Protein descriptionRecombinant fragment, corresponding to amino acids 1319-1710 of Human CBP, with an N-terminal enzyme tag, MW ~71kDa.
  • Expression hostE. coli

    • Properties

  • Purification notesPurity : >90% by densitometry.
  • FormLiquid
  • Storage instructionsShipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 25% Glycerol, 50mM Tris HCl, 150mM Sodium chloride, 0.25mM DTT, 0.1mM PMSF, pH 7.5
  • Concentration information loading...
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab56272 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    SDS-PAGE SDS-PAGE: Use at an assay dependent dilution.
  • Application notesThis peptide can be used with studies using ab137333.

    • Protein info

    • Alternative names
        CBPCBP/p300CBP_HUMAN
        CREB binding proteinCREB-binding proteinCREBBPCyclic AMP responsive enhancer binding proteinKAT3ARSTSRTSRubinstein Taybi syndrome
      see all
  • FunctionAcetylates histones, giving a specific tag for transcriptional activation. Also acetylates non-histone proteins, like NCOA3 coactivator. Binds specifically to phosphorylated CREB and enhances its transcriptional activity toward cAMP-responsive genes. Acts as a coactivator of ALX1 in the presence of EP300.
  • Involvement in diseaseNote=Chromosomal aberrations involving CREBBP may be a cause of acute myeloid leukemias. Translocation t(8;16)(p11;p13) with MYST3/MOZ; translocation t(11;16)(q23;p13.3) with MLL/HRX; translocation t(10;16)(q22;p13) with MYST4/MORF. MYST3-CREBBP may induce leukemia by inhibiting RUNX1-mediated transcription.
    Defects in CREBBP are a cause of Rubinstein-Taybi syndrome type 1 (RSTS1) [MIM:180849]. RSTS1 is an autosomal dominant disorder characterized by craniofacial abnormalities, broad thumbs, broad big toes, mental retardation and a propensity for development of malignancies.
  • Sequence similaritiesContains 1 bromo domain.
    Contains 1 KIX domain.
    Contains 2 TAZ-type zinc fingers.
    Contains 1 ZZ-type zinc finger.
  • DomainThe KIX domain mediates binding to HIV-1 Tat.
  • Post-translational
    modifications    Methylation of the KIX domain by CARM1 blocks association with CREB. This results in the blockade of CREB signaling, and in activation of apoptotic response.
    Phosphorylated upon DNA damage, probably by ATM or ATR.
    Sumoylation negatively regulates transcriptional activity via the recruitment of DAAX.
  • Cellular localizationCytoplasm. Nucleus. Recruited to nuclear bodies by SS18L1/CREST. In the presence of ALX1 relocalizes from the cytoplasm to the nucleus.

    • Target information above from: UniProt accession Q92793 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

    KAT3A / CBP protein (Tagged) images

    • SDS-PAGE - CBP protein (Tagged) (ab56272)
      SDS-PAGE - CBP protein (Tagged) (ab56272)
      ab56272 on SDS-PAGE.

    References for KAT3A / CBP protein (Tagged) (ab56272)

    ab56272 has not yet been referenced specifically in any publications.

    Publishing research using ab56272 ? Please let us know so that we can cite the reference in this datasheet

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    Votes: Highest First

    Sequence is different from the original product ab56272, which was requested to be active and DTT free. This protein shows no activity, because the active center is missing in this sequence!
    Further on, the Glutathione is unacceptable for her as well ...

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    Vielen Dank für Ihren Anruf und Ihre Geduld.

    Zu meiner großen Überraschung erhielt ich heute dann doch einmal eine Rückmeldung von dem Labor bezüglich des verpatzten Proteins: Die Verwirrung entstand anhand eines Datenbl...

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    Aktives und DTT freies KAT3A / CBP Protein

    Ich freue mich Ihnen nun mitteilen zu können dass wir das DTT freie KAT3A / CBP Protein nun im Katalog haben:

    ab135297

    http://www.abcam.com/index.html?datasheet=135297 (or use the following: http://www.abcam.com/index.html?datashe...

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    Aktives und DTT freies KAT3A / CBP Protein

    Vielen Dank für Ihren Anruf.

    Bitte entschuldigen Sie die Verzögerung in meiner Antwort.

    Wir sind momentan dabei das KAT3A / CBP Protein ohne DTT in unseren Katalog aufzunehmen.

    Ich werde Sie mit der Artikelnummer ko...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"