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Anti-CBS antibody (ab54883)

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Overview

Product name

Anti-CBS antibody
See all CBS products (4) ...

Description

Mouse monoclonal to CBS

Tested applications

WB, IHC-P, ICC/IFmore details

Cross reactivity

Reacts with

Human

Immunogen

Recombinant fragment: MPSETPQAEV GPTGCPHRSG PHSAKGSLEK GSPEDKEAKE PLWIRPDAPS RCTWQLGRPA SESPHHHTAP AKSPKILPDI LKKIGDTPMV RINKIGKKFG , corresponding to amino acids 1-101 of Human CBS

MPSETPQAEV GPTGCPHRSG PHSAKGSLEK GSPEDKEAKE PLWIRPDAPS RCTWQLGRPA SESPHHHTAP AKSPKILPDI LKKIGDTPMV RINKIGKKFG

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: None
PBS, pH 7.2

Concentration

Concentration information loading...

Purity

Protein G purified

Clonality

Monoclonal

Isotype

IgG2a

Light chain type

kappa

  • IHC-P - CBS antibody (ab54883)IHC-P - CBS antibody (ab54883) image (enlarge)

  • Western blot - CBS antibody (ab54883)Western blot - CBS antibody (ab54883) image (enlarge)

  • Immunocytochemistry/ Immunofluorescence-CBS antibody(ab54883)Immunocytochemistry/ Immunofluorescence-CBS antibody(ab54883) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab54883 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Target

Function

Only known pyridoxal phosphate-dependent enzyme that contains heme. Important regulator of hydrogen sulfide, especially in the brain, utilizing cysteine instead of serine to catalyze the formation of hydrogen sulfide. Hydrogen sulfide is a gastratransmitter with signaling and cytoprotective effects such as acting as a neuromodulator in the brain to protect neurons against hypoxic injury.

Tissue specificity

In the adult strongly expressed in liver and pancreas, some expression in heart and brain, weak expression in lung and kidney. In the fetus, expressed in brain, liver and kidney.

Pathway

Amino-acid biosynthesis; L-cysteine biosynthesis; L-cysteine from L-homocysteine and L-serine: step 1/2.

Involvement in disease

Defects in CBS are the cause of cystathionine beta-synthase deficiency (CBSD) [MIM:236200]. CBSD is an enzymatic deficiency resulting in altered sulfur metabolism and homocystinuria. The clinical features of untreated homocystinuria due to CBS deficiency include myopia, ectopia lentis, mental retardation, skeletal anomalies resembling Marfan syndrome, and thromboembolic events. Light skin and hair can also be present. Biochemical features include increased urinary homocystine and methionine.

Sequence similarities

Belongs to the cysteine synthase/cystathionine beta-synthase family.
Contains 1 CBS domain.

Cellular localization

Cytoplasm. Nucleus.

Target information above from: UniProt accessionP35520 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • Beta thionase antibody
  • Beta-thionase antibody
  • Cbs antibody
  • CBS_HUMAN antibody
  • Cystathionine beta synthase antibody
  • Cystathionine beta-synthase antibody
  • EC 4.2.1.22 antibody
  • HIP 4 antibody
  • HIP4 antibody
  • Methylcysteine synthase antibody
  • OTTHUMP00000109416 antibody
  • OTTHUMP00000109418 antibody
  • Serine sulfhydrase antibody
see all

Anti-CBS antibody images:

  IHC-P - CBS antibody (ab54883)

IHC-P - CBS antibody (ab54883)

CBS antibody (ab54883) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human hepatocellular carcinoma.

  Western blot - CBS antibody (ab54883)

Western blot - CBS antibody (ab54883)

CBS antibody (ab54883) at 1ug/lane + HeLa cell lysate at 25ug/lane.

  Immunocytochemistry/ Immunofluorescence-CBS antibody(ab54883)

Immunocytochemistry/ Immunofluorescence-CBS antibody(ab54883)

ICC/IF image of ab54883 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab54883, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96879, DyLight® 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-CBS antibody (ab54883)

ab54883 has not yet been referenced specifically in any publications.

Publishing research using ab54883? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"