Anti-CCDC6 antibody (ab85923)
Key features and details
- Rabbit polyclonal to CCDC6
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-CCDC6 antibody
See all CCDC6 primary antibodies -
Description
Rabbit polyclonal to CCDC6 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Dog, Pig, Xenopus laevis, Chimpanzee, Zebrafish, Rhesus monkey, Orangutan, Xenopus tropicalis, Medaka fish -
Immunogen
Synthetic peptide corresponding to a region between residues 75 and 125 of Human CCDC6 (AAC60637.1).
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Positive control
- HeLa whole cell lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab85923 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/2000 - 1/10000. Predicted molecular weight: 66 kDa.
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IP |
Use at 2-5 µg/mg of lysate.
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Notes |
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WB
1/2000 - 1/10000. Predicted molecular weight: 66 kDa. |
IP
Use at 2-5 µg/mg of lysate. |
Target
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Tissue specificity
Ubiquitously expressed. -
Involvement in disease
Defects in CCDC6 are a cause of thyroid papillary carcinoma (TPC) [MIM:188550]. TPC is a common tumor of the thyroid that typically arises as an irregular, solid or cystic mass from otherwise normal thyroid tissue. Papillary carcinomas are malignant neoplasm characterized by the formation of numerous, irregular, finger-like projections of fibrous stroma that is covered with a surface layer of neoplastic epithelial cells. Note=A chromosomal aberration involving CCDC6 is found in thyroid papillary carcinomas. Inversion inv(10)(q11.2;q21) generates the RET/CCDC6 (PTC1) oncogene. -
Domain
The protein has mostly an alpha helical conformation similar to myosin heavy-chain tail that might adopt a coiled-coil conformation. -
Cellular localization
Cytoplasm. Cytoplasm > cytoskeleton. May be a cytoskeletal protein. - Information by UniProt
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Database links
- Entrez Gene: 8030 Human
- Entrez Gene: 76551 Mouse
- Entrez Gene: 691155 Rat
- Omim: 601985 Human
- SwissProt: Q16204 Human
- SwissProt: D3YZP9 Mouse
- Unigene: 591360 Human
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Alternative names
- CCDC 6 antibody
- CCDC6 antibody
- CCDC6_HUMAN antibody
see all
Images
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All lanes : Anti-CCDC6 antibody (ab85923) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Developed using the ECL technique.
Predicted band size: 66 kDa
Observed band size: 70 kDa why is the actual band size different from the predicted?
Exposure time: 30 seconds -
Detection of Human CCDC6 in Immunoprecipitates of Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded) using ab85923 at 3 µg/mg lysate for IP and at 1 µg/ml for subsequent Western blot detection. Detection: Chemiluminescence with an exposure time of 10 seconds.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab85923 has not yet been referenced specifically in any publications.