Anti-CCK4 antibody (ab62073)

Overview

• Product nameAnti-CCK4 antibodySee all CCK4 primary antibodies ...
• Description
  Rabbit polyclonal to CCK4
• Tested applicationsICC/IF, WB, IHC-P more details
• Species reactivity
  Reacts with: Mouse, Rat, Human
  
• Immunogen

  Raised against an 18 amino acid peptide from near the carboxy terminus of human PTK7.

• Positive controlHuman colon, mouse kidney or rat liver membrane lysate

Properties

• FormLiquid
• Storage instructionsStore at +4°C.
• Storage bufferPreservative: 0.02% Sodium Azide
  Constituents: PBS
• Concentration information loading...
• PurityImmunogen affinity purified
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Metabolism
  • Types of disease
  • Obesity

  • Signal Transduction
  • Protein Phosphorylation
  • Tyrosine Kinases
  • Other

  • Tags & Cell Markers
  • Cell Type Markers
  • Tumor Associated

Applications

Target

• FunctionInactive tyrosine kinase involved in Wnt signaling pathway. Component of both the non-canonical (also known as the Wnt/planar cell polarity signaling) and the canonical Wnt signaling pathway. Functions in cell adhesion, cell migration, cell polarity, proliferation, actin cytoskeleton reorganization and apoptosis. Has a role in embryogenesis, epithelial tissue organization and angiogenesis.
• Tissue specificityHighly expressed in lung, liver, pancreas, kidney, placenta and melanocytes. Weakly expressed in thyroid gland, ovary, brain, heart and skeletal muscle. Also expressed in erythroleukemia cells. But not expressed in colon.
• Sequence similaritiesBelongs to the protein kinase superfamily. Tyr protein kinase family. Insulin receptor subfamily.
  Contains 7 Ig-like C2-type (immunoglobulin-like) domains.
  Contains 1 protein kinase domain.
• DomainThe protein kinase domain is predicted to be catalytically inactive.
• Post-translational
  modificationsMMP14 cleaves PTK7 between Pro-621 and Leu-622 generating an N-terminal soluble (70 kDa) fragment and a membrane C-terminal (50 kDa) fragment. Proteolysis by MMP14 regulates PTK7 function in non-canonical Wnt signaling pathway.
• Cellular localizationMembrane. Cell junction. Co-localizes with MMP14 at cell junctions. Also localizes at the leading edge of migrating cells.

Target information above from: UniProt accession Q13308 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 5754 Human
  • Entrez Gene: 71461 Mouse
  • Entrez Gene: 301242 Rat
  • Omim: 601890 Human
  • SwissProt: Q13308 Human
  • SwissProt: Q8BKG3 Mouse
  • Unigene: 90572 Human
  • Unigene: 181833 Mouse

  • Unigene: 198822 Rat

  see all

• Alternative names
  8430404F20Rik antibodyCCK 4 antibodyCCK-4 antibody

  Colon carcinoma kinase 4 antibodyHuman colon carcinoma kinase 4 (CCK4) mRNA complete cds antibodyInactive tyrosine-protein kinase 7 antibodymPTK7/CCK4 antibodyOTTHUMP00000217016 antibodyProtein tyrosine kinase PTK 7 antibodyProtein-tyrosine kinase 7 antibodyPseudo tyrosine kinase receptor 7 antibodyPTK 7 antibodyPTK7 antibodyPTK7 protein tyrosine kinase 7 antibodyPTK7 protein tyrosine kinase antibodyPTK7_HUMAN antibodySerum response factor antibodySrf antibodyTyrosine-protein kinase-like 7 antibody

  see all

Anti-CCK4 antibody images

• 

  Western blot - CCK4 antibody (ab62073)
  All lanes : Anti-CCK4 antibody (ab62073) at 1 µg/ml
  
  Lane 1 : Human colon tissue lysate
  Lane 2 : Mouse kidney tissue lysate
  Lane 3 : Rat liver tissue lysate
  
  Lysates/proteins at 10 µg per lane.
  
  Secondary
  anti-rabbit IgG
  
  Predicted band size : 86 kDa
  Observed band size : 86 kDa
  
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - CCK4 antibody (ab62073)

  ab62073 staining CCK4 in human Colon tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded sections).

• 

  Immunocytochemistry/ Immunofluorescence-Anti-CCK4 antibody(ab62073)
  Immunofluorescence of PTK7 in Human Colon cells using ab62073 at 20 ug/ml.