Recombinant Anti-CCL27 antibody [EPR8582(2)] (ab186421)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8582(2)] to CCL27
- Suitable for: Flow Cyt (Intra), WB, ICC/IF
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-CCL27 antibody [EPR8582(2)]
See all CCL27 primary antibodies -
Description
Rabbit monoclonal [EPR8582(2)] to CCL27 -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), WB, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Human CCL27 recombinant protein; A431 and HACAT cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR8582(2) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab186421 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
Flow Cyt (Intra) |
1/320.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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WB |
1/1000 - 1/10000. Detects a band of approximately 10 kDa (predicted molecular weight: 13 kDa).
|
|
ICC/IF |
1/250.
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Notes |
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Flow Cyt (Intra)
1/320. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
WB
1/1000 - 1/10000. Detects a band of approximately 10 kDa (predicted molecular weight: 13 kDa). |
ICC/IF
1/250. |
Target
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Function
Chemotactic factor that attracts skin-associated memory T-lymphocytes. May play a role in mediating homing of lymphocytes to cutaneous sites. Binds to CCR10. -
Tissue specificity
Testis, thymus, placenta, ovary and skin. -
Sequence similarities
Belongs to the intercrine beta (chemokine CC) family. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 10850 Human
- Entrez Gene: 100039863 Mouse
- Entrez Gene: 100039939 Mouse
- Entrez Gene: 100040048 Mouse
- Entrez Gene: 20301 Mouse
- Entrez Gene: 362505 Rat
- Omim: 604833 Human
- SwissProt: Q9Y4X3 Human
see all -
Alternative names
- ALP antibody
- C-C motif chemokine 27 antibody
- CC chemokine ILC antibody
see all
Images
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Immunofluorescent analysis of 4% paraformaldehyde-fixed A431 cells labeling CCL27 with ab186421 at 1/250 dilution followed by Goat anti rabbit IgG (Alexa Fluor® 555) secondary antibody at 1/200 dilution. Counter stained with Dapi.
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Anti-CCL27 antibody [EPR8582(2)] (ab186421) at 1/5000 dilution + Human CCL27 recombinant protein at 0.01 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 13 kDa -
Intracellular flow cytometric analysis of 2% paraformaldehyde-fixed HACAT cells labeling CCL27 with ab186421 at 1/320 dilution (red) compared to a Rabbit monoclonal IgG isotype control (green), followed by Goat anti rabbit IgG (FITC) secondary antibody at 1/150 dilution.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (3)
ab186421 has been referenced in 3 publications.
- Fu X et al. Qi-Long-Tian formula extract alleviates symptoms of acute high-altitude diseases via suppressing the inflammation responses in rat. Respir Res 22:52 (2021). PubMed: 33579290
- Tao B et al. CYB561D2 up-regulation activates STAT3 to induce immunosuppression and aggression in gliomas. J Transl Med 19:338 (2021). PubMed: 34372858
- Li S et al. HOXC10 promotes proliferation and invasion and induces immunosuppressive gene expression in glioma. FEBS J 285:2278-2291 (2018). PubMed: 29676849