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Anti-CCNL1 antibody (ab34709)

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Overview

Product name

Anti-CCNL1 antibody
See all CCNL1 products (5) ...

Description

Rabbit polyclonal to CCNL1

Specificity

This antibody is specific for human Cyclin L1a and shows negligible reactivity to Cyclin L1 beta or Cyclin L2 isoforms.

Tested applications

ELISA, WB, IHC-P, IP, ICC/IFmore details

Cross reactivity

Reacts with

Human

Predicted to work with

Mouse, Rat, Chicken, Dog, Chimpanzee

Immunogen

Synthetic peptide: KLKAKGL NP DGTPALST L GGFSPASKP SSPREVKAEE KSPISINVK Q, corresponding to amino acids 314-369 of Human CCNL1

KLKAKGL NPDGTPALST LGGFSPASKP SSPREVKAEE KSPISINVKT VKKEPEDRQ

Run BLAST with BLAST the sequence with ExPASyRun BLAST with BLAST the sequence with NCBI

Positive control

For Western blotting : Mouse brain whole cell lysate. For IHC: A variety of tissues including breast, heart, kidney [distal tubules], liver, skeletal muscle, spleen.

Properties

Form

Liquid

Storage instructions

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: 0.01% Sodium Azide
Constituents: 0.15M Sodium chloride, 0.02M Potassium phosphate. pH 7.2

Concentration

Concentration information loading...

Purity

Protein A purified

Clonality

Polyclonal

Isotype

IgG

Research areas

Epigenetics and Nuclear Signaling >> DNA / RNA >> RNA Processing >> Other
Epigenetics and Nuclear Signaling >> DNA / RNA >> RNA Processing >> Splicing

  • Immunohistochemistry (Formalin-fixed paraffin-embedded sections) - CCNL1 antibody (ab34709)Immunohistochemistry (Formalin-fixed paraffin-embedded sections) - CCNL1 antibody (ab34709) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab34709 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • ELISA

     

  • WB

     
  • 1 Image

    IHC-P

     

  • IP

     

  • ICC/IF

     

Application notes

ELISA: 1/5000 - 1/25,000.
ICC/IF: Use at an assay dependent dilution.
IHC-P: Use at a concentration of 10 - 20 µg/ml.
IP: Use at an assay dependent dilution.
WB: 1/2000 - 1/5000. Predicted molecular weight: 59 kDa. Approximately 35µg of lysate was separated by 4-20% SDS-PAGE followed by transfer to nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1:3,500 for 2h at room temperature followed by washes and reaction with a 1:10,000 dilution of IRDye™800 conjugated Gt-a-Rabbit IgG [H&L] for 45 min at room temperature.

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Target

Function

Transcriptional regulator which participates in regulating the pre-mRNA splicing process. Seems to be involved in the regulation of RNA polymerase II (pol II). Functions in association with cyclin-dependent kinases (CDKs) and has a role in the second step of splicing. May be a candidate proto-oncogene in head and neck squamous cell carcinomas (HNSCC). Inhibited by the CDK-specific inhibitor p21.

Tissue specificity

Ubiquitous with higher level in thymus. Overexpression in primary tumors of head and neck squamous cell carcinomas (HNSCC).

Sequence similarities

Belongs to the cyclin family. Cyclin L subfamily.

Domain

Contains a RS region (arginine-serine dipeptide repeat) within the C-terminal domain which is the hallmark of the SR family of splicing factors. This region probably plays a role in protein-protein interactions.

Cellular localization

Nucleus speckle. More specifically found in nuclear intrachromatin granules clusters (IGC), also called nuclear speckles, which are storage compartments for nuclear proteins involved in mRNA processing.

Target information above from: UniProt accessionQ9UK58 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • Ania 6a antibody
  • BM 001 antibody
  • CCNL 1 antibody
  • CCNL1 antibody
  • CCNL1_HUMAN antibody
  • Cyclin L ania 6a antibody
  • Cyclin L antibody
  • Cyclin L gamma antibody
  • Cyclin L1 antibody
  • Cyclin-L antibody
  • Cyclin-L1 antibody
see all

Database links

see all

Anti-CCNL1 antibody images:

  Immunohistochemistry (Formalin-fixed paraffin-embedded sections) - CCNL1 antibody (ab34709)

Immunohistochemistry (Formalin-fixed paraffin-embedded sections) - CCNL1 antibody (ab34709)

This image shows human spleen stained with ab34709 at 10µg/ml

References for Anti-CCNL1 antibody (ab34709)

ab34709 has not yet been referenced specifically in any publications.

Publishing research using ab34709? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"