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Products:Immunology >> Innate Immunity >> Macrophage / Inflamm.
Overview
Product name
Anti-CCR2 antibody
See all CCR2 products (6) ...
Description
Goat polyclonal to CCR2
Specificity
This antibody recognizes mouse CCR2 and the peptide sequence is < 50 % identical to other chemokine receptors in this region.
Tested applications
Flow Cyt, WB, ICC, IHC-Pmore details
Cross reactivity
Reacts with
Mouse
Immunogen
Synthetic peptide: SHSLFTRSIQELDEGATTPYDYDDGEPC, corresponding to N terminal amino acids 18-45 of Mouse CCR2
SHSLFTRSIQ ELDEGATTPY DYDDGEPC
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.1% Sodium Azide
Constituents: 1mg/ml BSA, 140mM Sodium chloride, 10mM Potassium phosphate. pH 7.2
Concentration
Concentration information loading...
Purity
Protein G purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cardiovascular >> Atherosclerosis >> Vascular Inflammation >> Leukocyte recruitment >> Chemokines
Cancer >> Signal transduction >> G protein signaling >> GPCR
Signal Transduction >> Signaling Pathway >> G Protein Signaling >> GPCR
Signal Transduction >> Signaling Pathway >> Nuclear Signaling >> STATs
Microbiology >> Interspecies Interaction >> Host Virus Interaction
Immunology >> Innate Immunity >> Chemokines >> Beta Chemokine Rec. (CCR)
Immunology >> Innate Immunity >> Macrophage / Inflamm.
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Immunohistochemistry (Paraffin-embedded sections) - CCR2 antibody (ab25788)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab25788 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Flow Cyt: Use at an assay dependent dilution.
WB: 1/1000Predicted molecular weight: 42 kDa.
ICC
ICC: 1/400ICC: 1/400
IHC-P: 1/250(We recommend using the heat mediated antigen retrieval method using citrate buffer pH 6.0, steaming for 20 minutes followed by cooling for 20 minutes.)
Target
Relevance
CCR2, or C C chemokine receptor type 2, is a receptor for several monocyte chemo attractant proteins (MCP1, MCP3, MCP4) which specifically mediate monocyte chemotaxis. CCR2 transduces such signals by increasing the intracellular level of calcium ions. For example, MCP1 is involved in monocyte infiltration in inflammatory diseases such as rheumatoid arthritis as well as in the inflammatory response against tumors. CCR2 is also an alternative coreceptor with CD4 for HIV1 infection. CCR2 has two isoforms and has been reported to be expressed in a wide variety of tissues, including blood, brain, heart, kidney, liver, lung, ovary, pancreas, spinal cord, spleen and thymus.
Cellular localization
Cell membrane; Multi-pass membrane protein
Alternative names
- Monocyte chemoattractant protein 1 receptor antibody
- C C chemokine receptor type 2 antibody
- C C CKR 2 antibody
see all
Database links
- Entrez Gene: 12772 Mouse
- SwissProt: P51683 Mouse
Anti-CCR2 antibody images:
Immunohistochemistry (Paraffin-embedded sections) - CCR2 antibody (ab25788)
Immunohistochemistry of CCR2 (ab25788) antibody to mouse CCR2 on paraffin section of mouse spleen.
References for Anti-CCR2 antibody (ab25788)
This product has been referenced in:
- U'Ren Let al. Type I interferons inhibit the generation of tumor-associated macrophages. Cancer Immunol Immunother 59:587-98 (2010). IHC-Fr; Mouse.Read more (PubMed: 19826812) »
- Noto Det al. In vitro differentiation of lineage-negative bone marrow cells into microglia-like cells. Eur J Neurosci 31:1155-63 (2010). Flow Cyt; Mouse.Read more (PubMed: 20345919) »
See all 6 publications for this product
Publishing research using ab25788? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"