CCR2 antibody (ab25788)

Product Name 

CCR2 antibody

Product type 

Primary antibodies

Description 

Goat polyclonal to CCR2

Immunogen 

Synthetic peptide: SHSLFTRSIQELDEGATTPYDYDDGEPC, corresponding to N terminal amino acids 18-45 of Mouse CCR2
BLAST 'SHSLFTRSIQELDEGATTPYDYDDGEPC' with or

Reacts with 
(species key)

Ms

Specificity 

This antibody recognizes mouse CCR2 and the peptide sequence is < 50 % identical to other chemokine receptors in this region.

Tested applications 
(see key)

Flow Cyt, ICC, IHC-P, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
Flow Cyt: Use at an assay dependent dilution (PMID 19917684).
ICC: 1/400.
IHC-P: 1/250. We recommend using the heat mediated antigen retrieval method using citrate buffer pH 6.0, steaming for 20 minutes followed by cooling for 20 minutes.
WB: 1/1000. Predicted molecular weight: 42 kDa.


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Cellular localization 

Membrane; multi pass membrane protein.

Research areas 

Cardiovascular >> Atherosclerosis >> Vascular Inflammation >> Leukocyte recruitment >> Chemokines
Cancer >> Signal transduction >> G protein signaling >> GPCR
Signal Transduction >> Signaling Pathway >> G Protein Signaling >> GPCR
Signal Transduction >> Signaling Pathway >> Nuclear Signaling >> STATs
Microbiology >> Interspecies Interaction >> Host Virus Interaction
Immunology >> Innate Immunity >> Chemokines >> Beta Chemokine Rec. (CCR)
Immunology >> Innate Immunity >> Macrophage / Inflamm.

Relevance 

CCR2, or C C chemokine receptor type 2, is a receptor for several monocyte chemo attractant proteins (MCP1, MCP3, MCP4) which specifically mediate monocyte chemotaxis. CCR2 transduces such signals by increasing the intracellular level of calcium ions. For example, MCP1 is involved in monocyte infiltration in inflammatory diseases such as rheumatoid arthritis as well as in the inflammatory response against tumors. CCR2 is also an alternative coreceptor with CD4 for HIV1 infection. CCR2 has two isoforms and has been reported to be expressed in a wide variety of tissues, including blood, brain, heart, kidney, liver, lung, ovary, pancreas, spinal cord, spleen and thymus.

Database links 

Raised in 

Goat

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Protein G purified

Storage buffer 

Preservative: 0.1% Sodium Azide
Constituents: 1mg/ml BSA, 140mM Sodium chloride, 10mM Potassium phosphate. pH 7.2

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

See below for CCR2 antibody images, references, products related to ab25788 and other tools.

CCR2 antibody images:

References for CCR2 antibody (ab25788)

  This product has been used in: (two most recent references)

Shao Z et al. Fms-Like Tyrosine Kinase 3 Ligand Regulates Migratory Pattern and Antigen Uptake of Lung Dendritic Cell Subsets in a Murine Model of Allergic Airway Inflammation. J Immunol 183:7531-7538 (2009). Flow Cyt; Mouse. PubMed: 19917684

Ishida Y et al. Chemokine receptor CX3CR1 mediates skin wound healing by promoting macrophage and fibroblast accumulation and function. J Immunol 180:569-79 (2008). IHC-P; Mouse. PubMed: 18097059

See all 3 publication references for this product.


If you publish research using ab25788 please let us know so that we can cite the reference on this datasheet.

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