Overview

  • Product nameAnti-CCR4 antibody
    See all CCR4 primary antibodies
  • Description
    Goat polyclonal to CCR4
  • SpecificityPeptide sequence is < 50 % identical to other human chemokine receptors in this region. The antibody recognizes mouse CCR4 and has no cross-reactivity to human CCR4.
  • Tested applicationsIHC-Fr, ELISA, ICC, WB, IHC-P, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse

    Does not react with

    Human
  • Immunogen

    Synthetic peptide:

    DTTQDETVYNSYYFYESMPC

    , corresponding to amino acids 8-26 of Mouse CCR4.

  • Positive control
    • Mouse spleen cells

Properties

Applications

Our Abpromise guarantee covers the use of ab1664 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent dilution.
ELISA 1/500000.
ICC 1/500.
WB 1/1000.
IHC-P Use at an assay dependent dilution.
Flow Cyt Use at an assay dependent dilution.

Target

  • FunctionHigh affinity receptor for the C-C type chemokines CCL17/TARC and CCL22/MDC. The activity of this receptor is mediated by G(i) proteins which activate a phosphatidylinositol-calcium second messenger system. Can function as a chemoattractant homing receptor on circulating memory lymphocytes and as a coreceptor for some primary HIV-2 isolates. In the CNS, could mediate hippocampal-neuron survival.
  • Tissue specificityPredominantly expressed in the thymus, in peripheral blood leukocytes, including T-cells, mostly CD4+ cells, and basophils, and in platelets; at lower levels, in the spleen and in monocytes. Detected also in macrophages, IL-2-activated natural killer cells and skin-homing memory T-cells, mostly the ones expressing the cutaneous lymphocyte antigen (CLA). Expressed in brain microvascular and coronary artery endothelial cells.
  • Sequence similaritiesBelongs to the G-protein coupled receptor 1 family.
  • Post-translational
    modifications
    In natural killer cells, CCL22 binding induces phosphorylation on yet undefined Ser/Thr residues, most probably by beta-adrenergic receptor kinases 1 and 2.
  • Cellular localizationCell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • C C chemokine receptor type 4 antibody
    • C C CKR 4 antibody
    • C-C chemokine receptor type 4 antibody
    • C-C CKR-4 antibody
    • CC CKR 4 antibody
    • CC-CKR-4 antibody
    • CCR 4 antibody
    • CCR-4 antibody
    • CCR4 antibody
    • CCR4_HUMAN antibody
    • CD194 antibody
    • Chemokine (CC motif) receptor 4 antibody
    • Chemokine (CC motif) receptor 4 antibody
    • chemokine C C motif receptor 4 antibody
    • ChemR13 antibody
    • CKR4 antibody
    • CMKBR 4 antibody
    • CMKBR4 antibody
    • HGCN 14099 antibody
    • K5 5 antibody
    • K5-5 antibody
    • MGC88293 antibody
    see all

References for Anti-CCR4 antibody (ab1664)

This product has been referenced in:
  • Poppensieker K  et al. CC chemokine receptor 4 is required for experimental autoimmune encephalomyelitis by regulating GM-CSF and IL-23 production in dendritic cells. Proc Natl Acad Sci U S A 109:3897-902 (2012). Flow Cyt ; Mouse . Read more (PubMed: 22355103) »
  • Stutte S  et al. Requirement of CCL17 for CCR7- and CXCR4-dependent migration of cutaneous dendritic cells. Proc Natl Acad Sci U S A 107:8736-41 (2010). Flow Cyt ; Mouse . Read more (PubMed: 20421491) »

See all 12 Publications for this product

Product Wall

Thank you very much for your patience and for the details in which you have provided. I contacted the originator of ab1664 and below is the FACS protocl that they used. I hope this will be of use to you and if you continue to experience difficulty with...

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Thank you very much for your enquiry and patience. At this time, the only publication that we are currently aware of featuring the use of ab1664 is the Hoogewerf A et al. reference. We also don't have any images/figures to share at this time. If you ca...

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Cysteine molecules are commonly placed on the end of an immunising amino acid sequence. The reason for this is two fold: 1) It allows the peptide to be sulphur-linked to a suitable carrier molecule to provoke an immune response in the host animal. ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"