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Product name
Anti-CCR4 antibody
See all CCR4 products (7) ...
Description
Goat polyclonal to CCR4
Specificity
Peptide sequence is < 50 % identical to other human chemokine receptors in this region.
Tested applications
Flow Cyt, ICC/IF, ELISA, ICC, IHC-P, WB, IHC-Frmore details
Cross reactivity
Reacts with
Mouse, Human, Cynomolgus Monkey
Immunogen
Synthetic peptide: SNYYLYESIPKPCTKEGIKAFGE, corresponding to amino acids 17-39 of Human CCR4 (extracellular domain).
SNYYLYESIPKPCTKEGIKAFGE
Positive control
Human peripheral blood cells or paraffin sections of spleen.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
PBS, 1mg/ml BSA, 0.1% sodium azide, pH7.2
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cardiovascular >> Atherosclerosis >> Vascular Inflammation >> Leukocyte recruitment >> Chemokines
Immunology >> Innate Immunity >> Chemokines >> Beta Chemokine Rec. (CCR)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab1669 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Flow Cyt: Use at an assay dependent concentration.
ICC/IF: Use at an assay dependent dilution. Fix cells with 4% PFA (see Ritter et al).
ELISA
ELISA: 1/100000.ELISA: 1/100000.
ICC
ICC: 1/300.ICC: 1/300.
IHC-P: 1/300.
WB: 1/1000. Detects a band of approximately 55 kDa (predicted molecular weight: 42 kDa).
IHC-Fr: Use at an assay dependent dilution. Fix in acetone at -20°C for 5 minutes. See Heller et al.
Target
Function
High affinity receptor for the C-C type chemokines CCL17/TARC and CCL22/MDC. The activity of this receptor is mediated by G(i) proteins which activate a phosphatidylinositol-calcium second messenger system. Can function as a chemoattractant homing receptor on circulating memory lymphocytes and as a coreceptor for some primary HIV-2 isolates. In the CNS, could mediate hippocampal-neuron survival.
Tissue specificity
Predominantly expressed in the thymus, in peripheral blood leukocytes, including T-cells, mostly CD4+ cells, and basophils, and in platelets; at lower levels, in the spleen and in monocytes. Detected also in macrophages, IL-2-activated natural killer cells and skin-homing memory T-cells, mostly the ones expressing the cutaneous lymphocyte antigen (CLA). Expressed in brain microvascular and coronary artery endothelial cells.
Sequence similarities
Belongs to the G-protein coupled receptor 1 family.
Post-translational
modifications
In natural killer cells, CCL22 binding induces phosphorylation on yet undefined Ser/Thr residues, most probably by beta-adrenergic receptor kinases 1 and 2.
Cellular localization
Cell membrane.
Target information above from: UniProt accessionP51679
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Chemokine (CC motif) receptor 4 antibody
- C C chemokine receptor type 4 antibody
- C C CKR 4 antibody
see all
Database links
- Entrez Gene: 1233 Human
- Entrez Gene: 12773 Mouse
- Omim: 604836 Human
- SwissProt: P51679 Human
- SwissProt: P51680 Mouse
- Unigene: 184926 Human
- Unigene: 1337 Mouse
Anti-CCR4 antibody images:
Western blot - CCR4 antibody (ab1669)
Anti-CCR4 antibody (ab1669) at 1 µg/ml +
Secondary
Rabbit polyclonal to Goat IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 42 kDa
Observed band size : 55 kDa (why is the actual band size different from the predicted?)
Additional bands at : 100 kDa,37 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 12 minutes
CCR4 contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCR4 antibody (ab1669)
IHC image of CCR4 staining in human spleen formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab1669, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Flow Cytometry - Anti-CCR4 antibody (ab1669)
ab1669 staining CCR4 in Mouse whole blood by Flow Cytometry. Red blood cells were lysed in PBS + 1% BSA and 0.01% sodium azide and fixed in paraformaldehyde. The sample was incubated with the primary antibody (1/100 in PBS + 1% BSA and 0.01% sodium azide) for 1 hour at 4°C. A biotin-conjugated Donkey anti-goat IgG polyclonal (1/200) was used as the secondary antibody.
Gating Strategy: Live Lymphocytes.
This image is courtesy of an anonymous Abreview
References for Anti-CCR4 antibody (ab1669)
This product has been referenced in:
- Song CH et al. Identification of chemoattractive factors involved in the migration of bone marrow-derived mesenchymal stem cells to brain lesions caused by prions. J Virol 85:11069-78 (2011).Read more (PubMed: 21813601) »
- Li JY et al. The chemokine receptor CCR4 promotes tumor growth and lung metastasis in breast cancer. Breast Cancer Res Treat : (2011). WB, IHC-P; Human.Read more (PubMed: 21479551) »
See all 6 publications for this product
Publishing research using ab1669? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"