CCR5 antibody [E164] (ab32048)

Product Name 

CCR5 antibody [E164]

Product type 

Primary antibodies

Description 

Rabbit monoclonal [E164] to CCR5

Immunogen 

A synthetic peptide corresponding to residues in C-terminus (cytoplasmic domain) of human CKR5

Reacts with 

Hu, Ms

Does not react with 
(species key)

Rat

Specificity 

ab32048 recognises CKR5/CCR5. It does not cross react with other G-protein coupled receptor 1 family members.

Tested applications 
(see key)

Flow Cyt, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
Flow Cyt: 1/20.
WB: 1/2000. Detects a band of approximately 60 kDa (predicted molecular weight: 41 kDa).

Is unsuitable for ICC, IHC or IP.


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

Jurkat, K562, Daudi cell lysates

Cellular localization 

Cell membrane; Multi-pass membrane protein

Research areas 

Cardiovascular >> Atherosclerosis >> Vascular Inflammation >> Leukocyte recruitment >> Chemokines
Cancer >> Signal transduction >> G protein signaling >> GPCR
Signal Transduction >> Signaling Pathway >> G Protein Signaling >> GPCR
Microbiology >> Interspecies Interaction >> Host Virus Interaction
Immunology >> Innate Immunity >> Chemokines >> Beta Chemokine Rec. (CCR)
Immunology >> Innate Immunity >> Macrophage / Inflamm.
Neuroscience >> Neurology process >> Neurodegenerative disease >> Alzheimer's disease >> Other

Relevance 

The CC chemokine receptor 5 (CCR5) is a member of the CC-chemokine receptor family, and has the characteristic structure of a 7 transmembrane G protein-coupled receptor (GPCR). CCR5 regulates trafficking and effector functions of memory/effector Th1 cells, macrophages, NK cells, and immature dendritic cells. CCR5 and its ligands play an important role in viral pathogenesis. CCR5 represents the co-receptor for macrophage (M) and dual (T cell and M)-tropic immunodeficiency viruses. Together with the CD4 binding receptor, CCR5 plays a critical role in HIV entry into the target cells. Moreover, the CCR5 ligands macrophage inflammatory protein (MIP)-1 alpha, MIP-1 beta and RANTES act as endogenous inhibitors of HIV infection, making both CCR5 and its chemokine ligands attractive therapeutic targets for HIV infection. Recent studies have also highlighted the role of CCR5 in a variety of other human diseases, ranging from infectious and inflammatory diseases to cancer.

Database links 

Raised in 

Rabbit

Clonality 

Monoclonal

Clone number 

E164

Isotype 

IgG

Purity 

IgG fraction

Storage buffer 

Preservative: 0.01% Sodium Azide
Constituents: 40% Glycerol, 0.05% BSA, 0.15M Sodium chloride, 50mM Tris glycine. pH 7.4

Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS

Form 

Liquid

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Notes 

Produced under U.S. Patent No. 5,675,063.

See below for CCR5 antibody [E164] images, references, products related to ab32048 and other tools.

CCR5 antibody [E164] images:

References for CCR5 antibody [E164] (ab32048)

  This product has been used in:

Ren H et al. High-level production, solubilization and purification of synthetic human GPCR chemokine receptors CCR5, CCR3, CXCR4 and CX3CR1. PLoS One 4:e4509 (2009). WB; Human. PubMed: 19223978

If you publish research using ab32048 please let us know so that we can cite the reference on this datasheet.

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