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Products:Neuroscience >> Neurology process >> Neurodegenerative disease >> Alzheimer's disease >> Other
Overview
Product name
Anti-CCR5 antibody
See all CCR5 products (25) ...
Description
Goat polyclonal to CCR5
Specificity
This antibody binds to CCR 5 receptors on human peripheral blood leukocytes as determined by immunocytochemistry.
Tested applications
IHC-Fr, WB, IHC-P, ICCmore details
Cross reactivity
Reacts with
Human, Macaque Monkey
Immunogen
Synthetic peptide: YQVSSPIYDINYYTSEPCQKINVKQIAA, corresponding to N terminal amino acids 3-30 of Human CCR5.
YQVSSPIYDI NYYTSEPCQK INVKQIAA
Positive control
Human peripheral blood cells or paraffin sections of spleen.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
PBS, 1mg/ml BSA, 0.1% sodium azide, pH7.2
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Immunology >> Immune System Diseases >> Antiviral Signaling >> HIV-related
Cardiovascular >> Atherosclerosis >> Vascular Inflammation >> Leukocyte recruitment >> Chemokines
Cancer >> Signal transduction >> G protein signaling >> GPCR
Signal Transduction >> Signaling Pathway >> G Protein Signaling >> GPCR
Microbiology >> Interspecies Interaction >> Host Virus Interaction
Immunology >> Innate Immunity >> Chemokines >> Beta Chemokine Rec. (CCR)
Immunology >> Innate Immunity >> Macrophage / Inflamm.
Neuroscience >> Neurology process >> Neurodegenerative disease >> Alzheimer's disease >> Other
-
- CCR5 antibody (ab1673)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab1673 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-Fr: Use at an assay dependent dilution.
WB
WB: 1/1000WB: 1/1000
IHC-P: 1/300(with biotinylated secondary and streptavidin-HRP. )
ICC
ICC: 1/300ICC: 1/300
Target
Function
Receptor for a number of inflammatory CC-chemokines including MIP-1-alpha, MIP-1-beta and RANTES and subsequently transduces a signal by increasing the intracellular calcium ion level. May play a role in the control of granulocytic lineage proliferation or differentiation. Acts as a coreceptor (CD4 being the primary receptor) for HIV-1 R5 isolates.
Tissue specificity
Highly expressed in spleen, thymus, in the myeloid cell line THP-1, in the promyeloblastic cell line KG-1A and on CD4+ and CD8+ T-cells. Medium levels in peripheral blood leukocytes and in small intestine. Low levels in ovary and lung.
Involvement in disease
Genetic variation in CCR5 is associated with suseptibility to diabetes mellitus insulin-dependent type 22 (IDDM22) [MIM:612522]. A multifactorial disorder of glucose homeostasis that is characterized by susceptibility to ketoacidosis in the absence of insulin therapy. Clinical fetaures are polydipsia, polyphagia and polyuria which result from hyperglycemia-induced osmotic diuresis and secondary thirst. These derangements result in long-term complications that affect the eyes, kidneys, nerves, and blood vessels.
Sequence similarities
Belongs to the G-protein coupled receptor 1 family.
Post-translational
modifications
Sulfated on at least 2 of the N-terminal tyrosines. Sulfation contributes to the efficiency of HIV-1 entry and is required for efficient binding of the chemokines, CCL3 and CCL4.
O-glycosylated, but not N-glycosylated. Ser-6 appears to be the major site. Also sialylated glycans present which contribute to chemokine binding. Thr-16 and Ser-17 may also be glycosylated and, if so, with small moieties such as a T-antigen.
Palmitoylation in the C-terminal is important for cell surface expression, and to a lesser extent, for HIV entry.
Phosphorylation on serine residues in the C-terminal is stimulated by binding CC chemokines especially by APO-RANTES.
Cellular localization
Cell membrane.
Target information above from: UniProt accessionP51681
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- AM4 7 antibody
- C C chemokine receptor type 5 antibody
- C C CKR 5 antibody
see all
Database links
- Entrez Gene: 1234 Human
- Omim: 601373 Human
- SwissProt: P51681 Human
- Unigene: 450802 Human
Anti-CCR5 antibody images:
References for Anti-CCR5 antibody (ab1673)
This product has been referenced in:
- Zimmermann Tet al. Low expression of chemokine receptor CCR5 in human colorectal cancer correlates with lymphatic dissemination and reduced CD8+ T-cell infiltration. Int J Colorectal Dis 25:417-24 (2010). WB, IHC-P; Human.Read more (PubMed: 20054600) »
- Tan MCet al. Disruption of CCR5-dependent homing of regulatory T cells inhibits tumor growth in a murine model of pancreatic cancer. J Immunol 182:1746-55 (2009). IHC-P; Human.Read more (PubMed: 19155524) »
See all 4 publications for this product
Publishing research using ab1673? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"